Influence of Drug-Containing Serum of Huangqi [Astragalus membranaceus（ Fisch. ） Bunge ], Shuizhi（ Hirudo） and Their Formulation on the Expression of NF-кB,MMP-2,Fas/Fas L in Glomerular Mesangial Cells in Rats

WANG Guangyang; REN Xianzhi

doi:10.13288/j.11-2166/r.2015.01.016

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Influence of Drug-Containing Serum of Huangqi [Astragalus membranaceus（ Fisch. ） Bunge ], Shuizhi（ Hirudo） and Their Formulation on the Expression of NF-кB,MMP-2,Fas/Fas L in Glomerular Mesangial Cells in Rats

更新时间：2021-11-17

- Influence of Drug-Containing Serum of Huangqi [Astragalus membranaceus（ Fisch. ） Bunge ], Shuizhi（ Hirudo） and Their Formulation on the Expression of NF-кB,MMP-2,Fas/Fas L in Glomerular Mesangial Cells in Rats
- Journal of Traditional Chinese Medicine Vol. 56, Issue 1, Pages: 59-62(2015)
- 作者机构：
  
  1. 南京中医药大学第一临床医学院
  2. 南京中医药大学附属江苏省中医院
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2015.01.016
  CLC： R285.5
- Published：2015
- 稿件说明：
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[1]王广洋,任现志.黄芪、水蛭及其配方含药血清对大鼠肾小球系膜细胞NF-кB、MMP-2、Fas、FasLmRNA表达的影响[J].中医杂志,2015,56(01):59-62.

WANG Guangyang, REN Xianzhi. Influence of Drug-Containing Serum of Huangqi [Astragalus membranaceus（ Fisch. ） Bunge ], Shuizhi（ Hirudo） and Their Formulation on the Expression of NF-кB,MMP-2,Fas/Fas L in Glomerular Mesangial Cells in Rats[J]. Journal of Traditional Chinese Medicine2015, 56(1): 59-62.
[1]王广洋,任现志.黄芪、水蛭及其配方含药血清对大鼠肾小球系膜细胞NF-кB、MMP-2、Fas、FasLmRNA表达的影响[J].中医杂志,2015,56(01):59-62. DOI： 10.13288/j.11-2166/r.2015.01.016.

WANG Guangyang, REN Xianzhi. Influence of Drug-Containing Serum of Huangqi [Astragalus membranaceus（ Fisch. ） Bunge ], Shuizhi（ Hirudo） and Their Formulation on the Expression of NF-кB,MMP-2,Fas/Fas L in Glomerular Mesangial Cells in Rats[J]. Journal of Traditional Chinese Medicine2015, 56(1): 59-62. DOI： 10.13288/j.11-2166/r.2015.01.016.

摘要

目的研究黄芪、水蛭及其配方含药血清干预系膜增生性肾小球肾炎的可能作用机制。方法 20只SD大鼠随机分为黄芪高血清组、水蛭高血清组、黄芪高水蛭高血清组、黄芪低水蛭高血清组

每组5只。大鼠给药量5 g/（kg·d）为低浓度

2倍剂量为高浓度

各组均配成0.5 g/ml混悬液

每天早晚各灌胃1次

连续灌胃5天

末次灌胃1.5 h后制备含药血清。检测各组不同浓度（10%、20%、40%、80%）的含药血清对大鼠系膜细胞增殖的影响

计算细胞存活率

筛选出各组最佳浓度。系膜细胞以1×105个/孔接种于6孔板中

每孔2 ml

分为正常组、模型组、黄芪高血清组、水蛭高血清组、黄芪高水蛭高血清组、黄芪低水蛭高血清组

除正常组外其余各组加入脂多糖诱导大鼠肾小球系膜细胞增殖

再分别加入筛选出的不同浓度的含药血清进行干预

检测各组系膜细胞中核转录因子（NF-кB）、基质金属蛋白酶2（MMP-2）、Fas、Fas L mRNA转录水平。结果黄芪高血清组、水蛭高血清组、黄芪高水蛭高血清组、黄芪低水蛭高血清组含药血清最佳浓度分别为10%、20%、40%、20%。与正常组比较

模型组系膜细胞中NF-кB、MMP-2、Fas、Fas L mRNA表达明显升高（P<0.05）

而各含药血清组NF-кB、MMP-2、Fas、Fas L mRNA表达较模型组显著降低（P<0.01）。结论黄芪、水蛭及其配方含药血清可抑制大鼠系膜细胞增殖

其作用机制可能与下调NF-кB、MMP-2、Fas、Fas L mRNA表达水平有关。

Abstract

Objective To study the possible mechanism of containing serum of Huangqi [Astragalus membranaceus（ Fisch.） Bunge]

) and their formulation on mesangial proliferative glomerulonephritis（ Ms PGN）. Methods Twenty SD rats were randomly assigned to high-dose Huangqi serum group

high-dose Shuizhi serum group

high-dose Huangqi and Shuizhi serum group

low-dose Huangqi and high-dose Shuizhi serum group with 5 rats in each group.The concentration of 5 g /（ kg·d） was defined as low-dose

while 10 g /（ kg·d） as high-dose. Suspension of 0. 5g / ml was prepared to apply on the mornings and evenings by gavage everyday for 5 days. Drug-containing serum was prepared 1. 5 h after the last gavage. Influence on cell proliferation was investigated in different concentration（ 10%

20%

40%

80%） of drug-containing serums and cell survival rate was calculated to scree

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