Objective To observe the impact of Chinese medicines with function to tonify qi and nourish yin（ YQYY）

strengthen spleen and warm kidney（ JPWS）

and soften hardness and disperse nodules（ RJSJ） on subcutaneous tumor transplanted from human lung cancer NCI-H460 cells in nude mice

and to discuss its possible mechanism. Methods The human non-small cell lung cancer（ NSCLC） cell line H460 was inoculated subcutaneously into 32 nude mice. After tumor formation

all mice were randomly divided into a JPWS group

a YQYY group

a RJSJ group

and a saline group

with 8 in each. The concentration of JPWS

YQYY

and RJSJ were 1. 1 g / ml

1. 9g / ml

and 1. 8 g / ml

respectively. One month after inoculation all mice were given corresponding medicinals by gastric administration

0. 3 ml each time

once a day

for 15 days. Tumor volume and weight in tumor-bearing mice were measured

inhibition rate was calculated

and expression of MicroRNA21 and MicroRNA218 was detected by PCR in each group. Results On Day 10 after administration

the tumor volume of mice in all group increased significantly compared with before medication

and on Day 15 the tumor volume of mice in the YQYY group

RJSJ group

and saline group increased compared with before treatment（ P < 0. 05）. The tumor inhibition rates in the JPWS group

YQYY group

and RJSJ group were 9. 9%

16. 3%

and 11. 8%

respectively

with no significant differences among groups（ P > 0. 05）. The tumor weight and MicroRNA-21 expression levels in the YQYY group were significantly lower than that in the saline group（ P < 0. 05）. Conclusion Medicines with the effect of tonifying qi and nourishing yin can inhibit tumor growth in nude mice. Down-regulating the expression of oncogenes related MicroRNA may be one of the possible mechanisms.