Objective To explore the modeling methods of animal models of Defensive-qi deficiency syndrome

and to observe the disproof effect of Yupingfeng San on the rat models. Methods Fifty sprague-dawley rats were randomized into normal control group（ group A）

cyclophosphamide group（ group B）

cold and hot stimulation group（group C）

fatigue with cold and hot stimulation group（group D）

and Chinese Traditional Medicine group（group E）

with ten rats in each group. In group A

rats were fed routinely; in group B

10 mg / kg of cyclophosphamide was intraperitoneal injected to the rats every day; cold and hot stimulation was given to the rats once a day in group C;and made rats swim to fatigue

and then gave cold and hot stimulation once daily to the rats in group D and group E.In addition

Yupingfeng San 25 g / kg was given to rats in group E once day. Each group was treated for seven days.After then general condition and behavior-expressing of rats were observed in each group

the similarity between the results by each modeling method and Weiqi deficiency syndrome was estimated

and the body weight

thymus index

spleen index

phagocytic function of extracorporeal macrophage

and spleen levels of interleukin-2（ IL-2） and interleukin-6（ IL-6） were compared between groups. Result It was found in behavior-expressing of rats that the model of fatigue with cold and hot stimulation in Group D was more close to the macroscopic evaluation standard of Weiqi deficiency syndrome. Compared with group A

body weight of rats reduced significantly in group B

D

and E（ P <0. 05）. Compared with group C

body weight of rats reduced significantly in group D（ P < 0. 05）. Compared with group D

body weight of rats increased remarkably in group E（ P < 0. 05）. In addition

compared with group A

thymus index and spleen index

phagocytic rate and phagocytic index of macrophage

and levels of IL-2 and IL-6reduced significantly in group B

C

D

and E（ P < 0. 05）. Compared with group D

thymus index and spleen index

phagocytic rate and phagocytic index of macrophage

and levels of IL-2 and IL-6 increases significantly in group E（ P < 0. 05）. Conclusion Fatigue with cold and hot stimulation is a preferable method to establish rat model with Weiqi deficiency syndrome. This conclusion can be disproved by Yupingfeng San.