Objective To study the effect and the possible mechanism of ligustrazine on the adhesion of PG-BE1 stem like cells in the pulmonary giant cell carcinoma of human with high metastatic lung cancer. Methods The stem like cells were sorted from PG-BE1 cells by no serum ball culture

the expression of OCT-4

SOX-2

Nanog protein and the flat panel clone formation experiment were tested for verification. Then artificial basement membrane adhesion experiment was taken to compare the difference of adhesion between PG-BE1 stem like cells and its primary cells in different environments（ normal oxygen and hypoxia）. At the same time

the adhesion ability of stem like cells in each group with intervention of different concentrations of ligustrazine（ final concentrations of 100

300

500 g / ml）and cisplatin（ 2 g / ml） were observed

the surface adhesion molecules beta integrin and the expression of CD44V6 in the stem like cells in each group were also observed after intervention. Results Compared with the primary cells

the PG-BE1 stem cells like cells were more capable of forming clone foci. The expression of SOX-2 OCT-4 and Nanog protein in the surface of stem like cells was stronger（ P < 0. 05 or P < 0. 01）. The adhesion ability of the normal oxygen stem like cells and the hypoxia stem like cells was significantly higher than that of the primary cells（ P < 0. 5or P < 0. 01）. The adhesion ability of the stem like cells in ligustrazine groups with middle and high dose and cisplatin groups was lower than that in control group（ P < 0. 01）. The rate of cell adhesion inhibition was up to 42. 2% in the ligustrazine group with high dose. The expression of beta integrin and CD44V6 in the stem like cells of normal oxygen and hypoxia was higher than those of the primary cells（ P < 0. 01）. The expression of beta integrin in groups of ligustrazine with low

middle and high dose and cisplatin group was lower than that of control group（ P < 0. 01）. The expression of CD44V6 in the high dose group of ligustrazine was significantly lower than that in the control group（ P <0. 01）. Conclusion The adhesion of PG-BE1 stem like cells to the basement membrane is stronger than that of its primary cells. Ligustrazine can inhibit its adhesion ability; the underlying mechanism may be related to the inhibition of the expression of the integrin and CD44V6 protein in the cell surface.