Objective To explore the effects and possible mechanism of sequential intervention of kidneyreinforcing and blood-activating on endometrium regeneration of menstruation model mice. Methods Totally 140 female C57BL /6J mice were randomly divided into sham-operated group（ n = 40）

model group（ n = 50）

sequential interventional group（ n = 50）. Model group and sequential interventional group adopted hormone sequential processing after removing bilateral ovaries of mice to copy menstruation model of mice. Sequential interventional group was given kidney-reinforcing decoction and blood-activating decoction sequentially while adopting hormone sequential processing

perfused with corresponding medicine 2 ml /100 g

for 14 days. Model group and sham-operated group were perfused with equivalent distilled water. Conducted morphological observation and scoring on guiding lateral（ right） uterine horn of mice in each group 24 h

32 h

40 h

48 h

72 h after the withdrawal of progesterone

and detected the expression of Cxcl 2 protein of mice uteri. Results The morpholigical score of mice endometrium in model group decreased remarkably at 24 h

32 h

40 h

48 h compared with that of sham-operated group（ P < 0. 05）; The morpholigical score of mice endometrium in sequential interventional group increased remarkably at 24 h

32 h

40 h

48 h compared with that of model group（ P < 0. 05）. The expression of Cxcl 2 protein of model group all increased at any time point compared with that of sham-operated group（ P < 0. 05）; The expression of Cxcl 2 protein of sequential interventional group all increased at any time point compared with that of model group（ P < 0. 05）. Conclusion Sequential intervention of kidney-reinforcing and blood-activating can improve endometrium regeneration of menstruation model mice

and the underlying mechanism may be related to regulating the expression of Cxcl 2 protein.