Influence of Kangxianling Decoction（ 抗 纤 灵 方 ） on Expression of Matrix Metalloproteinase 2 and Tissue Inhibitor of Metalloproteinase 1 mRNA in Renal Tissue of Mice with 5 /6 Nephrectomy

ZHONG Liping; MA Zhiheng; YU Kena; HE Liqun

doi:10.13288/j.11-2166/r.2015.16.020

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Influence of Kangxianling Decoction（抗纤灵方） on Expression of Matrix Metalloproteinase 2 and Tissue Inhibitor of Metalloproteinase 1 mRNA in Renal Tissue of Mice with 5 /6 Nephrectomy

更新时间：2021-11-17

- Influence of Kangxianling Decoction（抗纤灵方） on Expression of Matrix Metalloproteinase 2 and Tissue Inhibitor of Metalloproteinase 1 mRNA in Renal Tissue of Mice with 5 /6 Nephrectomy
- Journal of Traditional Chinese Medicine Vol. 56, Issue 16, Pages: 1425-1428(2015)
- 作者机构：
  
  上海中医药大学附属曙光医院
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2015.16.020
  CLC： R285.5
- Published：2015
- 稿件说明：
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[1]钟利平,麻志恒,余柯娜,何立群.抗纤灵方对5/6肾切小鼠肾组织中基质金属蛋白酶2及基质金属蛋白酶组织抑制因子1 mRNA表达的影响[J].中医杂志,2015,56(16):1425-1428.

ZHONG Liping, MA Zhiheng, YU Kena, et al. Influence of Kangxianling Decoction（抗纤灵方） on Expression of Matrix Metalloproteinase 2 and Tissue Inhibitor of Metalloproteinase 1 mRNA in Renal Tissue of Mice with 5 /6 Nephrectomy[J]. Journal of Traditional Chinese Medicine2015, 56(16): 1425-1428.
[1]钟利平,麻志恒,余柯娜,何立群.抗纤灵方对5/6肾切小鼠肾组织中基质金属蛋白酶2及基质金属蛋白酶组织抑制因子1 mRNA表达的影响[J].中医杂志,2015,56(16):1425-1428. DOI： 10.13288/j.11-2166/r.2015.16.020.

ZHONG Liping, MA Zhiheng, YU Kena, et al. Influence of Kangxianling Decoction（抗纤灵方） on Expression of Matrix Metalloproteinase 2 and Tissue Inhibitor of Metalloproteinase 1 mRNA in Renal Tissue of Mice with 5 /6 Nephrectomy[J]. Journal of Traditional Chinese Medicine2015, 56(16): 1425-1428. DOI： 10.13288/j.11-2166/r.2015.16.020.

摘要

目的观察抗纤灵方抗肾纤维化的作用机理。方法 180只C57小鼠随机取出30只作为假手术组

其余150只采用5/6肾切除方法制作肾功能衰竭模型

造模成功后随机分为模型组、低剂量组、中剂量组、高剂量组、西药组

每组30只。假手术组及模型组给予0.5 ml自来水灌胃

低、中、高剂量组分别给予抗纤灵方水煎剂（药物剂量依次为0.2 g/kg、0.4 g/kg、0.8 g/kg）0.5 ml灌胃治疗

西药组给予雷帕霉素溶液（药物剂量0.0000016 g/kg）0.5 ml灌胃

每日灌胃1次

各组疗程均为12周。在灌胃后第4、8、12周末

每组分别取出10只小鼠处死

检测肾组织中基质金属蛋白酶2（MMP-2）、基质金属蛋白酶组织抑制因子1（TIMP-1）mRNA的表达。结果与假手术组比较

第4、8、12周末模型组小鼠MMP-2 mRNA、TIMP-1 mRNA表达水平均显著升高（P<0.01）;与模型组比较

在第8、12周末低、中、高剂量组和西药组MMP-2 mRNA表达水平均显著下降（P<0.05）

第4、8、12周末低、中、高剂量组TIMP-1 mRNA表达降低（P<0.05）;与低剂量组比较

高剂量组第8、12周末MMP-2 mRNA表达水平均明显下降（P<0.05）;与本组第4周末比较

低、中、高剂量组第8、12周末TIMP-1 mRNA表达水平均降低（P<0.05）。结论抗纤灵方抗肾纤维化的作用机理可能与抑制MMP-2 mRNA表达、TIMP-1 mRNA表达相关。

Abstract

Objective To observe the mechanism of anti-renal fibrosis of Kangxianling Decoction（抗纤灵方）.Methods Thirty mice were randomly assigned into sham-operated group from 180 C57 mice. The rest 150 mice received 5 /6 renal nephrectomy to establish renal failure model

and then they were equally assigned into model group

low dosage group

middle dosage group

high dosage group and western medicine group. Sham-operated group and model groups were given 0. 5 ml water. Low

middle and high dosage groups were given 0. 5 ml Kangxianling Decoction

0. 2 g / kg

0. 4 g / kg and 0. 8 g / kg

respectively. Western medicine group was given 0. 5 ml sirolimus solution（ 0. 0000016 g / kg）. All the treatments in every group were given by gavage once a day for 12 weeks. On the fourth

eighth and twelfth weekends

10 mice in each group were randomly selected to be executed. Expression of matrixmetalloproteinase 2（ MMP-2） and tissue inhibitor of metalloproteinase 1（ TIMP-1） mRNA in renal tissue were detected. Results Comparing with sham-operated group

levels of MMP-2 mRNA and TIMP-1 mRNA on the fourth

eighth and twelfth weekends in model group were significantly elevated（ P < 0. 01）. Levels of MMP-2 mRNA on the eighth and twelfth weekends in low

middle

high dosage and western medicine groups were significantly lower than those in model group（ P < 0. 05）; while levels of TIMP-1 mRNA on the fourth

eighth and twelfth weekends in low

middle

high dosage and western medicine groups were significantly lower than those in model group（ P <0. 05）. Expressions of MMP-2 mRNA on the eighth and twelfth weekends in high dosage group were significantly lower than those in low dosage group（ P < 0. 05）. In low

middle and high dosage groups

expressions of TIMP-1mRNA were significantly decreased on the eighth and twelfth weekends comparing with those on the fourth weekend（ P < 0. 05）. Conclusion The mechanism of anti-renal fibrosis of Kangxianling Decoction may be associated with inhibition of MMP-2 mRNA and TIMP-1 mRNA expression.

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