Objective To explore possible functional mechanism of Jianpi Qinghua Recipe treating type 2 diabetes mellitus at gene transcriptional level. Methods Randomly selected 40 from 50 Wistar rats to establish type 2diabetes mellitus model

with the other 10 rats taken as control group. Thirty successful modeling rats were randomly divided into model group and Jianpi Qinghua Recipe group

with 15 rats each. Perfused rats in Jianpi Qinghua Recipe group with Jianpi Qinghua Recipe 15 g /（ kg·d）. Perfused rats in model group with normal saline of the same capacity

conducting nothing on rats of control group. Intervention time of each group was all for 4 weeks. Tested levels of fast blood glucose（ FBG） of rats every week before and after treatment

and blood lipid level 4 weeks after treatment;Adopting Affymetrix gene chip technology to test differential expression gene of liver tissue of rats in each groups;Located signal pathways and gene related to Jianpi Qinghua Recipe affecting expression and regulating of level of transcription of livers of rats suffering from 2-diabetes mellitus

through gene chip signal pathways. Results FBG level of Jianpi Qinghua Recipe group decreased remarkably after treating for 1

2

3

4 weeks

and was lower than that of model group at the same time point（ P < 0. 05）; Blood lipid level of rats in Jianpi Qinghua Recipe group decreased compared with that of model group（ P < 0. 05 or P < 0. 01）. Jianpi Qinghua Recipe can remarkably affect several signal pathways

such as insulin secretion pathway

fatty acid metabolism pathway and TNF signal pathway

and can up-regulate the expression of PCIF1 and INSIG1 gene in the pathways. Conclusion Jianpi Qinghua Recipe may regulate gene transcriptional level of livers of rats suffering from type 2 diabetes mellitus by affecting gene expression of multiple signaling pathways

to increase glucose levels.