Effects of American Ginseng Capsules on the Liver Ultrastructure and the Oxidative Stress of Rats after Exposure to Electromagnetic Radiation

LUO Yaping; MA Huirong; CAO Xiaohui; SONG Cuimiao; MA Xuelian

doi:10.13288/j.11-2166/r.2015.18.017

您当前的位置：

首页 >

文章列表页 >

Effects of American Ginseng Capsules on the Liver Ultrastructure and the Oxidative Stress of Rats after Exposure to Electromagnetic Radiation

更新时间：2021-11-17

- Effects of American Ginseng Capsules on the Liver Ultrastructure and the Oxidative Stress of Rats after Exposure to Electromagnetic Radiation
- Journal of Traditional Chinese Medicine Vol. 56, Issue 18, Pages: 1593-1596(2015)
- 作者机构：
  
  1. 河北省中医院
  2. 河北中医学院
  3. 河北医科大学
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2015.18.017
  CLC： R285.5
- Published：2015
- 稿件说明：
移动端阅览
[1]罗亚萍,马惠荣,曹晓慧,宋翠淼,马雪莲.西洋参胶囊对电磁辐射大鼠肝脏超微结构及氧化应激的影响[J].中医杂志,2015,56(18):1593-1596.

LUO Yaping, MA Huirong, CAO Xiaohui, et al. Effects of American Ginseng Capsules on the Liver Ultrastructure and the Oxidative Stress of Rats after Exposure to Electromagnetic Radiation[J]. Journal of Traditional Chinese Medicine2015, 56(18): 1593-1596.
[1]罗亚萍,马惠荣,曹晓慧,宋翠淼,马雪莲.西洋参胶囊对电磁辐射大鼠肝脏超微结构及氧化应激的影响[J].中医杂志,2015,56(18):1593-1596. DOI： 10.13288/j.11-2166/r.2015.18.017.

LUO Yaping, MA Huirong, CAO Xiaohui, et al. Effects of American Ginseng Capsules on the Liver Ultrastructure and the Oxidative Stress of Rats after Exposure to Electromagnetic Radiation[J]. Journal of Traditional Chinese Medicine2015, 56(18): 1593-1596. DOI： 10.13288/j.11-2166/r.2015.18.017.

摘要

目的研究西洋参胶囊防治电磁辐射致大鼠肝损伤的可能作用机制。方法 40只SD大鼠随机分为正常组、辐射组、水飞蓟宾组、西洋参组各10只。除正常组外其余3组大鼠每天暴露于电磁辐射（900 MHz

370μW/cm2）4 h连续12天

辐射同时水飞蓟宾组、西洋参组大鼠每日灌服水飞蓟宾胶囊溶液（浓度为7 mg/ml）和西洋参胶囊溶液（浓度为19.7 mg/ml）1 ml/200 g

正常组、辐射组灌服等量生理盐水。12天后处死大鼠

透射电镜观察肝脏超微结构

检测肝组织丙二醛（MDA）含量和谷胱甘肽（GSH）活性

检测大鼠肝组织磷酸化的血管共济失调突变蛋白（ATM）和磷酸化组蛋白2A变异体（γH2AX）表达。结果与正常组比较

辐射组大鼠肝细胞线粒体和粗面内质网轻度受损

肝组织MDA含量升高

GSH活性降低

ATM和γH2AX表达明显升高（P<0.01）;与辐射组比较

水飞蓟宾组和西洋参组大鼠肝线粒体和粗面内质网受损减轻

肝组织MDA含量降低

GSH活性增加

ATM和γH2AX表达明显降低（P<0.05或P<0.01）。结论西洋参胶囊可改善电磁辐射致大鼠肝脏超微结构损伤

其机制可能与减轻氧化应激和降低ATM和γH2AX蛋白表达有关。

Abstract

Objective To study the possible mechanisms of American ginseng capsules in prevention and treatment of rat liver injury induced by electromagnetic radiation. Methods Forty Sprague-Dawley rats were randomly divided into normal group

radiation group

silibinin group

and American ginseng group

with ten in each group.Except the normal group

the three rest groups of rats were exposed to electromagnetic radiation（ 900 MHz

370 μW /cm2）

4 h per day

for 12 days in a row. At the same time

the silibinin group and the American ginseng group rats were administered orally with silibinin capsules solution（ 7 mg / ml） and American ginseng capsules solution（ 19. 7 mg /ml） 1 ml/200 g every day respectively. And the normal group and the radiation group were given with equivalent normal saline. After 12 days

the rats were killed and liver ultrastructure was observed under transmission electron microscope. The content of malondialdehyde（ MDA）

the activity of glutathione（ GSH）

and the expression of phosphorylated vascular ataxia mutation protein（ ATM） and phosphorylated histone 2A variants（ γH2AX） in liver tissue were also detected. Results Compared with the normal group

the mitochondria and rough endoplasmic reticulum of liver cells mildly damaged

the content of MDA in liver tissue increased

the activity of GSH decreased

and the expression of ATM and γH2AX increased significantly in the radiation group（ P < 0. 01）. Compared with the radiation group

the mitochondria and rough endoplasmic reticulum of liver cells showed less damage

the content of MDA in liver tissue decreased

the activity of GSH increased

and the expression of ATM and γH2AX decreased significantly in the silibinin group and the American ginseng group（ P < 0. 05 or P < 0. 01）. Conclusion American ginseng capsules can improve the ultrastructure injury of rat liver induced by electromagnetic radiation. Its mechanism may be related to reduction of oxidative stress and the protein expression of ATM and γH2AX.

关键词

Keywords

references

查看原文

Views

190

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of Erzhi Pill （二至丸） of Different Dosing Time on the Expression of Biological Clock-related Factors Bmal1 and Cry1 in the Liver Tissue of Aging Liver Injury Model Mice

Effect of Yiqi Yangyin Huoxue Formula（益气养阴活血方）on Oxidative Stress and Inflammatory Injury in Membranous Nephropathy Model Rats：Based on the NOX4-mediated SDF-1α/CXCR4 Signaling Pathway

Differentiation and Treatment Strategies for Pediatric IgA Vasculitis Based on the Correlation Between Blood Turbidity Theory and Oxidative Stress

Mechanisms of Hepatotoxicity Induced by Toxic Chinese Herbal Medicinals and Compatibility-Based Detoxification Strategies from the Perspective of the "Liver Aversion to Acute Irritation" Theory

Compound Tongluo Decoction（复方通络饮）in Treating Subcortical Ischemic Vascular Mild Cognitive Impairment with Liver-Kidney Deficiency and Phlegm-Stasis Obstructing Collateral Syndrome： A Randomized Controlled Trial

Related Author

MA Xiangyu

GONG Yuqing

WEI Xinyi

FENG Menglin

LI Sanqiang

DUAN Lengxin

GUO Xiaoxiao

SONG Chundong

Related Institution

School of Basic Medicine， Henan University of Science and Technology

College of Pediatrics，Henan University of Chinese Medicine

Department of Pediatrics，First Affiliated Hospital of Henan University of Chinese Medicine

Pediatric Medical College，Henan University of Chinese Medicine

Children Hospital，First Affiliated Hospital of Henan University of Chinese Medicine

Address：No.16, Nanxiao street, Dongzhimen, Dongcheng District, Beijing Postal code：100700
Tel：010-64035632 Email：jtcmcn@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19 京公网安备11010102007558号
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰