Effect of Zuogui Wan（左归丸） on Fetal Rats Birth Weight of Gestational Diabetes Mellitus Rats

WANG Yueyao; WANG Yonghui; LI Yanyan; CHAI Zhi; GAO Li; ZHOU Ran

doi:10.13288/j.11-2166/r.2015.19.017

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Effect of Zuogui Wan（左归丸） on Fetal Rats Birth Weight of Gestational Diabetes Mellitus Rats

更新时间：2021-11-17

- Effect of Zuogui Wan（左归丸） on Fetal Rats Birth Weight of Gestational Diabetes Mellitus Rats
- Journal of Traditional Chinese Medicine Vol. 56, Issue 19, Pages: 1682-1685(2015)
- 作者机构：
  
  1. 成都中医药大学基础医学院
  2. 山西中医学院
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2015.19.017
  CLC： R285.5
- Published：2015
- 稿件说明：
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[1]王悦尧,王永辉,李艳彦,柴智,高丽,周然.左归丸对妊娠糖尿病大鼠胎鼠出生体重的影响[J].中医杂志,2015,56(19):1682-1685.

WANG Yueyao, WANG Yonghui, LI Yanyan, et al. Effect of Zuogui Wan（左归丸） on Fetal Rats Birth Weight of Gestational Diabetes Mellitus Rats[J]. Journal of Traditional Chinese Medicine2015, 56(19): 1682-1685.
[1]王悦尧,王永辉,李艳彦,柴智,高丽,周然.左归丸对妊娠糖尿病大鼠胎鼠出生体重的影响[J].中医杂志,2015,56(19):1682-1685. DOI： 10.13288/j.11-2166/r.2015.19.017.

WANG Yueyao, WANG Yonghui, LI Yanyan, et al. Effect of Zuogui Wan（左归丸） on Fetal Rats Birth Weight of Gestational Diabetes Mellitus Rats[J]. Journal of Traditional Chinese Medicine2015, 56(19): 1682-1685. DOI： 10.13288/j.11-2166/r.2015.19.017.

摘要

目的观察左归丸对妊娠糖尿病大鼠胎鼠出生体重的影响及可能作用机制。方法 SD大鼠受孕后随机分为正常对照组、妊娠糖尿病组、胰岛素干预组、左归丸干预组、联合干预组

每组10只。受孕当日除正常对照组外其余各组大鼠腹腔注射链尿佐菌素45 mg/kg建立妊娠糖尿病大鼠模型。于妊娠第3天开始左归丸干预组、联合干预组给予左归丸10 g/（kg·d）灌胃

正常对照组和妊娠糖尿病组灌胃等量蒸馏水

每日1次;胰岛素干预组、联合干预组给予诺和灵R 20 IU/（kg·d）皮下注射

每日1次

至孕18天。孕19天解剖大鼠

称胎鼠体重

检测血清、皮质酮、胎盘11β-羟基类固醇脱氢酶2（11β-HSD2）mRNA和蛋白表达。结果与正常对照组比较

妊娠糖尿病组大鼠血清皮质酮水平明显升高

胎鼠体重、胎盘11β-HSD2 mRNA和蛋白表达降低（P<0.05或P<0.01）;与妊娠糖尿病组比较

胰岛素干预组、联合干预组大鼠血清皮质酮水平均明显降低

胎鼠体重升高（P<0.05或P<0.01）

左归丸干预组的胎鼠体重比较差异无统计学意义（P>0.05）

联合干预组胎盘11β-HSD2 mRNA和蛋白表达明显升高（P<0.05）。结论左归丸可以改善妊娠糖尿病病理状态对胎鼠体重的影响

联合胰岛素效更佳

其机制可能是通过上调11β-HSD2的表达、降低皮质酮浓度有关。

Abstract

Objective To observe the effect and possible mechanism of Zuogui Wan on fetal rats birth weight of gestational diabetes mellitus（ GDM） rats. Methods Pregnant SD rats were randomly divided into normal control group

GDM group

insulin intervention group

Zuogui Wan intervention group and combined intervention group

10 rats in each group. On the day of conception

rats of these groups were injected intraperitoneally with streptozocin 45 mg / kg to establish GDM rat models except rats of normal control group. From the third day of conception

Zuogui Wan intervention group and combined intervention group were given Zuogui Wan 10 g /（ kg·d） by gavage. Normal control group and GDM group were given equivalent distilled water by gavage

once each day. Insulin intervention group and combined intervention group were given Novolin R 20 IU /（ kg·d） by subcutaneous injection

once each day

until 18 days of conception. On the 19 th day of conception

rats were anatomized. Weigh fetal rats

detect serum corticosterone

placenta 11β-hydroxysteroid dehydrogenase 2（ 11β-HSD2） mRNA and the expression of protein.Results Compared with normal control group

serum corticosterone in GDM group increased; fetal rats weight

placenta11β-HSD2 mRNA and the expression of protein decreased（ P < 0. 05 or P < 0. 01）. Compared with GDM group

serum corticosterone in insulin intervention group and combined intervention group decreased; fetal rats weight increased（ P < 0. 05 or P < 0. 01）. Fetal rats weight in Zuogui Wan intervention group had no significant difference（ P > 0. 05）. Placenta11β-HSD2 mRNA and the expression of protein in combined intervention group increased（ P <0. 05）. Conclusion Zuogui Wan could improve the effect of GDM pathological state on fetal rats weight. It was more effective to combine with insulin. The mechanism may relate to up-regulate the expression of 11β-HSD2 and decrease the concentration of corticosterone.

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