SU Fei, SUN Yanan, CUI Xiaoyun, et al. The Effects of Yiqi Liangxue Shengji Formula(益气凉血生肌方) on Cell Morphology of Injured Vascular Endothelial Cells[J]. Journal of Traditional Chinese Medicine2015, 56(19): 1691-1694.
SU Fei, SUN Yanan, CUI Xiaoyun, et al. The Effects of Yiqi Liangxue Shengji Formula(益气凉血生肌方) on Cell Morphology of Injured Vascular Endothelial Cells[J]. Journal of Traditional Chinese Medicine2015, 56(19): 1691-1694.DOI: 10.13288/j.11-2166/r.2015.19.019.
The Effects of Yiqi Liangxue Shengji Formula(益气凉血生肌方) on Cell Morphology of Injured Vascular Endothelial Cells
Objective To study the mechanism of Yiqi Liangxue Shengji Formula in decreasing long term cardiovascular events after percutaneous coronary intervention from the view of vascular endothelial cell morphplogy.Methods Randomly divided 20 rabbits into normal group
normal saline group
Chinese medicine group
western medicine group and integrat medicine group
with 4 rabbits in each group. Perfused Chinese medicine group with Yiqi Liangxue Shengji Formula 2. 3 g /( kg·d)
western medicine group with losartan pills 0. 115 g /( kg·d)
Chinese and western medicine group respectively with equal dosage of Yiqi Liangxue Shengji Formula and losartan pills at 30-minutes intervals. Perfused normal saline group with equal dosage of Na Cl solution. No treatment was given to normal group. Collected blood sample from ear marginal veins after perfusing for 5 days continuously. Aseptically separated serum. Adopting angiotensin Ⅱ( Ang Ⅱ) to establish cell injury model on human umbilical vein endothelial cells( HUVECs)
and divided them into control group
model group
Chinese medicine group
Chinese and western medicine group. Added successively corresponding 0. 15 ml 15% medicated serum. Adopting inverted microscope to observe integral form changes of cells after culturing in incubator for 4 to 6 hours
and adopting transmission electron microscope to observe ultrastructure changes in cells. Results Under optical microscope
model group cells grew more slowly with more disordered form. After medicine intervention cells grow faster with form recovering
that of Chinese medicine and western medicine group was more remarkable. By transmission electron microscope
Chinese medicine group
western medicine group
Chinese and western medicine group injured vascular endothelial cells nucleus boundary was explicit
endoplasmic reticulum increased and densified
the number of mitochondrion increased
compared with model group. Chinese medicine group
Chinese and western medicine group endoplasmic reticulum fold more obvious
mitochondrion was full
with its number was more than western medicine group.Conclusion Yiqi Liangxue Shengji Formula has the function of promoting repairing the injured vascular endothelial cells in morphology. It can protect the integrity of endothelial cell
which can decrease the injury of coronary artery intervention therapy to endothelial cells.
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Related Author
LI Jinxia
FU Yimin
XU Yingzi
ZONG Wenjing
CAO Hongxin
LIU Wanghua
ZHENG Caixing
MA Zheng
Related Institution
Hunan University of Chinese Medicine
China Academy of Chinese Medical Sciences
China Association of Chinese Medicine
Dongzhimen Hospital,Beijing University of Chinese Medicine