Impact of Yifei Xuanfei Jiangzhuo Formula（益肺宣肺降浊方） on Expressions of Brain Tissues Hippocampus Calmodulin-dependent Protein Kinases Ⅱ and synapsin in Vascular Dementia Rats

WU Lin; ZHAO Haitao; CHEN Wei; ZHENG Fukui; ZENG Dongjuan; BAI Shuoyu; TANG Nong

doi:10.13288/j.11-2166/r.2015.21.018

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Impact of Yifei Xuanfei Jiangzhuo Formula（益肺宣肺降浊方） on Expressions of Brain Tissues Hippocampus Calmodulin-dependent Protein Kinases Ⅱ and synapsin in Vascular Dementia Rats

更新时间：2021-11-17

- Impact of Yifei Xuanfei Jiangzhuo Formula（益肺宣肺降浊方） on Expressions of Brain Tissues Hippocampus Calmodulin-dependent Protein Kinases Ⅱ and synapsin in Vascular Dementia Rats
- Journal of Traditional Chinese Medicine Vol. 56, Issue 21, Pages: 1871-1874(2015)
- 作者机构：
  
  1. 广西中医药大学
  2. 广西中医药大学第一附属医院
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2015.21.018
  CLC： R285.5
- Published：2015
- 稿件说明：
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[1]吴林,赵海涛,陈炜,郑福奎,曾东娟,白硕宇,唐农.益肺宣肺降浊方对血管性痴呆大鼠脑组织海马CaMPK-Ⅱ及突触蛋白表达的影响[J].中医杂志,2015,56(21):1871-1874.

WU Lin, ZHAO Haitao, CHEN Wei, et al. Impact of Yifei Xuanfei Jiangzhuo Formula（益肺宣肺降浊方） on Expressions of Brain Tissues Hippocampus Calmodulin-dependent Protein Kinases Ⅱ and synapsin in Vascular Dementia Rats[J]. Journal of Traditional Chinese Medicine2015, 56(21): 1871-1874.
[1]吴林,赵海涛,陈炜,郑福奎,曾东娟,白硕宇,唐农.益肺宣肺降浊方对血管性痴呆大鼠脑组织海马CaMPK-Ⅱ及突触蛋白表达的影响[J].中医杂志,2015,56(21):1871-1874. DOI： 10.13288/j.11-2166/r.2015.21.018.

WU Lin, ZHAO Haitao, CHEN Wei, et al. Impact of Yifei Xuanfei Jiangzhuo Formula（益肺宣肺降浊方） on Expressions of Brain Tissues Hippocampus Calmodulin-dependent Protein Kinases Ⅱ and synapsin in Vascular Dementia Rats[J]. Journal of Traditional Chinese Medicine2015, 56(21): 1871-1874. DOI： 10.13288/j.11-2166/r.2015.21.018.

摘要

目的探讨益肺宣肺降浊方治疗血管性痴呆的可能作用机制。方法 165只SD大鼠造模前随机选出24只作为假手术组

其余141只大鼠采用结扎双侧颈总动脉阻断血流法建立血管性痴呆模型

将造模成功的96只大鼠随机分为中药高、中、低剂量组和模型组、石杉碱甲组、脑复康组

每组16只。造模第15天开始灌胃给药

中药高、中、低剂量组分别给予益肺宣肺降浊方24.36、12.18、6.09 g/（kg·d）

石杉碱甲组、脑复康组分别给予石杉碱甲片、脑复康片0.15 mg/（kg·d）

模型组及假手术组均予等体积双蒸水。各组均灌胃30天后采用Western blot法检测大鼠脑组织海马钙调素依赖性蛋白激酶Ⅱ（Ca MPK-Ⅱ）及突触蛋白水平。结果模型组大鼠脑组织海马Ca MPK-Ⅱ、突触蛋白表达均明显低于假手术组（P<0.05）

石杉碱甲组、脑复康组和中药中、高剂量组Ca MPK-Ⅱ、突触蛋白表达均较模型组升高（P<0.05）

并且以中药高剂量组Ca MPK-Ⅱ升高最明显（P<0.05）。结论益肺宣肺降浊方可能通过上调脑组织海马Ca MPK-Ⅱ、突触蛋白表达

改善学习和记忆功能

从而治疗血管性痴呆。

Abstract

Objective To explore the possible mechanism of Yifei Xuanfei Jiangzhuo Formula（益肺宣肺降浊方）treating vascular dementia（ VD）. Methods Before modeling

24 rats among 165 SD rats were randomized into sham operation group. The other 141 rats were established VD models by the method of bilateral common carotid artery ligation to block blood circulation. Ninety-six successfully modeled rats were randomized into Chinese medicine high

middle and low dose groups

model group

huperzine A group and piracetam group

16 rats in each group. On the 15 th day of modeling

rats were given medicine by gavage. Chinese medicine high

middle and low dose groups were given Yifei Xuanfei Jiangzhuo Formula 24. 36

12. 18 and 6. 09 g /（ kg ·d） respectively. Huperzine A group and piracetam group were given Huperzine A and piracetam tablets 0. 15 mg /（ kg·d） respectively. Model group and sham operation group were given equivalent double distilled water. After 30 days of administration by gavage

detect the expressions of rats’ brain tissues hippocampus Calmodulin-dependent protein kinases Ⅱ（ Ca MPK-Ⅱ） and synapsin with western blot method. Results The expressions of brain tissues hippocampus Ca MPK-Ⅱ and synapsin in model group were lower than those in sham operation group（ P < 0. 05）. The expressions of Ca MPK-Ⅱand synapsin in Huperzine A group

piracetam group

Chinese medicine high

middle and low dose groups were higher than those in model group（ P < 0. 05）. The increase in Chinese medicine high dose group was the most significant（ P < 0. 05）.Conclusion Yifei Xuanfei Jiangzhuo Formula might up-regulate brain tissue hippocampus Ca MPK-Ⅱ and synapsin expressions and improve study and memorization function to treat VD.

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