Objective To observe the impact of Yishen Huoxue Fang on sperm quality of model rats with asthenospermia. Methods Fifty rats were randomized into normal group

model group and Chinese medicine high

middle and low dose groups

with 10 rats in each group. Rats were given 20 mg /（ kg·d） of tripterygium wilfordii hook tablets by gavage for 5 weeks to establish asthenospermia models except rats in normal group. After modeling successfully

Chinese medicine high

middle and low dose groups were given 15

10

or 5 g /（ kg · d） of Yishen Huoxue Fang by gavage for 5 weeks

respectively. Normal and model groups were fed normally. After the last administration

bilateral testis of rats were taken

weight and volume of bilateral testis

as well as weight of bilateral epididymides were measured

and density

viability and motility of sperm were detected. Results There is no significant difference in sperm density between all groups（ P>0. 05）. Compared with in normal group

sperm viability and sperm of A grade

B grade and（ A + B） grade decreased markedly in model group（ P<0. 05）. Compared with in model group

sperm viability and sperm of B grade and（ A + B） grade increased significantly in Chinese medicine low

middle and high dose groups（ P<0. 05）. There were no significant differences in weight and volume of bilateral testis

as well as epididymides weight between model group and Chinese medicine low

middle and high dose groups（ P>0. 05）. Conclusion Yishen Huoxue Fang could improve sperm viability and motility

but could not improve sperm density of rats with asthenospermia

and it has little effect on testical weight

testical volume and epididymides weight.