Effect of Tiaogan Lipi Fang（调肝理脾方） on the Regulation of T Cells and Liver Tissues in Mice With Primary Biliary Cirrhosis

GUO Xiaoxia; LI Liangxue; WU Yupeng; JIA Lili; LIU Gaiping; BAI Chongzhi

doi:10.13288/j.11-2166/r.2016.02.018

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Effect of Tiaogan Lipi Fang（调肝理脾方） on the Regulation of T Cells and Liver Tissues in Mice With Primary Biliary Cirrhosis

更新时间：2021-11-17

- Effect of Tiaogan Lipi Fang（调肝理脾方） on the Regulation of T Cells and Liver Tissues in Mice With Primary Biliary Cirrhosis
- Journal of Traditional Chinese Medicine Vol. 57, Issue 2, Pages: 161-165(2016)
- 作者机构：
  
  山西省中医院
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2016.02.018
  CLC： R285.5
- Published：2016
- 稿件说明：
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[1]郭晓霞,李良学,武玉鹏,贾力莉,刘改萍,白崇智.调肝理脾方对原发性胆汁性肝硬化小鼠调节性T细胞及肝组织病理的影响[J].中医杂志,2016,57(02):161-165.

GUO Xiaoxia, LI Liangxue, WU Yupeng, et al. Effect of Tiaogan Lipi Fang（调肝理脾方） on the Regulation of T Cells and Liver Tissues in Mice With Primary Biliary Cirrhosis[J]. Journal of Traditional Chinese Medicine2016, 57(2): 161-165.
[1]郭晓霞,李良学,武玉鹏,贾力莉,刘改萍,白崇智.调肝理脾方对原发性胆汁性肝硬化小鼠调节性T细胞及肝组织病理的影响[J].中医杂志,2016,57(02):161-165. DOI： 10.13288/j.11-2166/r.2016.02.018.

GUO Xiaoxia, LI Liangxue, WU Yupeng, et al. Effect of Tiaogan Lipi Fang（调肝理脾方） on the Regulation of T Cells and Liver Tissues in Mice With Primary Biliary Cirrhosis[J]. Journal of Traditional Chinese Medicine2016, 57(2): 161-165. DOI： 10.13288/j.11-2166/r.2016.02.018.

摘要

目的探讨调肝理脾方治疗原发性胆汁性肝硬化的可能作用机制。方法 120只C57BL/6小鼠随机分为正常组、模型组、阳性组和治疗组

每组30只。除正常组外其余各组应用聚肌胞苷酸腹腔注射（5 mg/kg

2次/周

共24周）建立原发性胆汁性肝硬化动物模型

造模同时阳性组给予熊去氧胆酸胶囊0.1 g/（kg·d）

治疗组给予调肝理脾方27 g/（kg·d）

正常组及模型组给予等体积生理盐水

每日灌胃1次

共24周。于实验第8、16、24周时检测各组小鼠血清抗线粒体抗体M

亚型（AMA-M2）阳性率、外周血CD4

CD25

Foxp3

调节性T细胞（Treg）细胞计数、肝组织病理学检查及肝组织细胞角蛋白19（CK19）表达。结果除正常组外

第8、16、24周时各组血清AMA-M

阳性率比较差异均无统计学意义（P

0.05）。与正常组比较

模型组小鼠第8、16、24周时外周血CD4

CD25

Foxp3

Treg细胞计数明显下降（P

0.05）;在第24周时

阳性组和治疗组外周血CD4

CD25

Foxp3

Treg细胞计数均较模型组升高（P

0.05）

且治疗组升高较阳性组明显（P

0.05）。与模型组比较

治疗组在第8、16、24周时肝组织病理分期差异有统计学意义（P

0.05）。与正常组比较

模型组第8、16、24周时CK19表达均明显升高（P

0.05）;与模型组比较

治疗组第24周时CK19表达降低（P

0.05）。结论调肝理脾方能够上调原发性胆汁性肝硬化模型小鼠外周血CD4

CD25

Foxp3

Treg细胞计数、降低肝组织CK19蛋白表达

从而改善胆小管损伤和肝组织纤维化。

Abstract

Objective To study the possible mechanism of Tiaogan Lipi Fang（调肝理脾方） in the treatment of primary biliary cirrhosis. Methods Totally 120 C57 BL /6 rats were randomly divided into normal group

model group

positive group and treatment group

with 30 rats in each group. Except the normal group

the rats in the other groups received intraperitoneal injection of poly I-poly（ C） 5 mg / kg

twice a week

for 24 weeks to establish the animal model of primary biliary cirrhosis. Meanwhile

the positive group was given Ursodeoxycholic Acid Capules 0. 1 g /（ kg·d）

the treatment group was given Tiaogan Lipi Fang 27g /（ kg·d）

the normal group and the model group were given equal volume of normal saline

all by gavage once a day for 24 weeks. At the 8th

16 th

and 24 th week of the experiment

the positive rate of anti mitochondrial antibody M

subtype（ AMA-M

）

peripheral blood CD4

CD25

Foxp3

regulatory T cell（ Treg） count

and liver tissue pathological examination and expression of liver tissue and cell keratin 19（ CK19） were detected in all rats. Results There was no significant difference in the positive rate of AMA-M2 between the model group

positive group and treatment group at the 8th

16 th and 24 th week（ P

0. 05）. Compared with the normal group

the number of CD4

CD25

Foxp3

Treg cells in peripheral blood in the model group was significantly decreased at 8th

16 th and 24 th week（ P

0. 05）; At the 24 th week

the number of CD4

CD25

Foxp3

Treg cells in peripheral blood in the positive group and the treatment group was higher than that in the model group

but the treatment group was significantly higher than that in the positive group（ P

0. 05）.There was significant difference in the pathological staging of liver tissues between the treatment group and the model group at the 8th

16 th and 24 th week（ P

0. 05）. Compared with the normal group

the expression of CK19 in the model group was much higher than that in normal group at the 8th

16 th and 24 th week（ P

0. 05）; Compared with the model group

CK19 expression was decreased in the treatment group at the 24 th week（ P

0. 05）. Conclusion Tiaogan Lipi Fang can up-regulate the activity of CD4

CD25

Foxp3

Treg in peripheral blood and reduce the expression of CK19 protein in liver tissue of rats model with primary liver cirrhosis

so as to improve the bile duct injury and hepatic fibrosis.

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Related Institution

Hennan Institute of Medical and Pharmaceutical Sciences，Zhengzhou University

The First Clinical Medical College of Henan University of Chinese Medicine

The First Affiliated Hospital of Henan University of Chinese Medicine

The Second Affliated Hospital of Guizhou University of Traditional Chinese Medicine

Guizhou University of Traditional Chinese Medicine

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