Objective To explore the possible mechanism of alum-borneol nanoemulsion for burn. Methods Epidermal stem cells were divided into low

middle and high dose herb groups

the normal group

as well as the control A and control B groups. Low

middle and high dose herb groups were given 1 ml of 8. 15

16. 3

or 32. 6 mg /L of alum-borneol nanoemulsion

respectively. The control A group was given 1 ml of moisture exposed burn ointment（ 湿润烧伤膏）. The control B group was given 1 ml of Defined K-SFM medium alone. The expression of epidermal stem cells osteopontin was detected with the method of Reverse Transcription Polymerase Chain Reaction（ RT-PCR）and immunoblotting at the time points of the 24 th hour

3rd day

5th day and 7th day. Results Results from RTPCR detecting showed that

compared with the control groups

the expression of osteopontin in middle and high dose herb groups decreased significantly at all time points（ P < 0. 05）

and it was lower than that in low dose herb group（ P < 0. 05）. Results of Western blot detecting showed that

compared with the control groups

the expression of osteopontin in middle and high dose herb groups decreased significantly on the 3rd

5th and 7th day（ P < 0. 05）.Compared with the low dose herb group

the expression of osteopontin in middle and high dose herb groups decreased significantly on the 3rd

5th and 7th day（ P < 0. 05）. Conclusion Alum-borneol nanoemulsion could regulate the expression of epidermal stem cells osteopontin gene. The most effective concentration is 8. 15 mg / L. It might relate to increasing the expression of osteopontin.