Objective To study possible mechanism of Yishen Huazhuo Fang（ 益肾化浊方） to treat Alzheimer’s disease. Methods The researchers extracted and cultured neural stem cells in vitro from Kunming mice with 14-day pregnancy. Then the researchers established neurovirulent cell models of Alzheimer’s disease mediated by soluble Aβ25- 35（ 5 μmol/L） in differentiation system. Samples were divided into the control group（ equivalent medium）

the model group（ Aβ25- 35

100 μl

5 μmol/L） and the Chinese medicine of Yishen Huazhuo group（ Aβ25- 35

100 μl

5 μmol / L + Yishen Huazhuo Fang 100 μl

0. 1 μg / ml）. After conventional digesting those cells and counting

the researchers inoculated them in 6-hole plates according to cell density 2 × 105/ ml（ 2 ml in per hole） and cultured for 48 h. The researchers used western blot method to detect expression of glial fibrillary acidic protein（ GFAP）

microtubulin（ Tubulin） and transcriptional activator 3（ STAT3）

phosphorylated transcriptional activator 3（ P-STAT3） and Smad1 protein. They also used RT-PCR method to detect gene expression of GFAP

STAT3

Smad1 and Tubulin. Results Compared with the control group

expression of GFAP

STAT3 gene and protein and P-STAT3 protein in the model group significantly increased. However expression of Tubulin gene and protein significantly decreased（ P < 0. 05）. Expression of Smad1 gene and protein unconspicuously increased（ P > 0. 05）.Compared with the model group

expression of GFAP

STAT3

Smad1 gene and protein and P-STAT3 protein in the Chinese medicine of Yishen Huazhuo group significantly decreased. However expression of Tubulin gene and protein significantly increased（ P < 0. 05）. Conclusion Yishen Huazhuo Fang may restrain neural stem cells from differentiation towards astrocytes through regulating expression of related proteins and gene in the JAK / STAT signaling pathway to delay the development of Alzheimer’s disease.