Effects of Medicated Serum with Yinqiao San（ 银翘散） with Different Decocting Time on Influenza A Virus Stimulated Macrophage Toll-like Receptors and Downstream My D88 Dependent and Independent Pathway

HUO Bingjie; CHANG Liang; LIU Yu; LI Mei; LIU Yaxian

doi:10.13288/j.11-2166/r.2016.07.016

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Effects of Medicated Serum with Yinqiao San（银翘散） with Different Decocting Time on Influenza A Virus Stimulated Macrophage Toll-like Receptors and Downstream My D88 Dependent and Independent Pathway

更新时间：2021-11-17

- Effects of Medicated Serum with Yinqiao San（银翘散） with Different Decocting Time on Influenza A Virus Stimulated Macrophage Toll-like Receptors and Downstream My D88 Dependent and Independent Pathway
- Journal of Traditional Chinese Medicine Vol. 57, Issue 7, Pages: 601-604(2016)
- 作者机构：
  
  河北医科大学第四医院
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2016.07.016
  CLC： R285
- Published：2016
- 稿件说明：
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[1]霍炳杰,常靓,刘羽,李梅,刘亚娴.不同煎煮时间银翘散含药血清对甲型流感病毒刺激巨噬细胞Toll样受体及其下游MyD88依赖和非依赖途径的影响[J].中医杂志,2016,57(07):601-604.

HUO Bingjie, CHANG Liang, LIU Yu, et al. Effects of Medicated Serum with Yinqiao San（银翘散） with Different Decocting Time on Influenza A Virus Stimulated Macrophage Toll-like Receptors and Downstream My D88 Dependent and Independent Pathway[J]. Journal of Traditional Chinese Medicine2016, 57(7): 601-604.
[1]霍炳杰,常靓,刘羽,李梅,刘亚娴.不同煎煮时间银翘散含药血清对甲型流感病毒刺激巨噬细胞Toll样受体及其下游MyD88依赖和非依赖途径的影响[J].中医杂志,2016,57(07):601-604. DOI： 10.13288/j.11-2166/r.2016.07.016.

HUO Bingjie, CHANG Liang, LIU Yu, et al. Effects of Medicated Serum with Yinqiao San（银翘散） with Different Decocting Time on Influenza A Virus Stimulated Macrophage Toll-like Receptors and Downstream My D88 Dependent and Independent Pathway[J]. Journal of Traditional Chinese Medicine2016, 57(7): 601-604. DOI： 10.13288/j.11-2166/r.2016.07.016.

摘要

目的探讨银翘散抗甲型流感病毒的最佳煎煮时间。方法将银翘散药粉分别制备成浓度为0.25（低）、0.50（中）、1.00 g/ml（高）的溶液

加热到100℃时开始计时

分别煎煮至3、6、12 min

4℃保存备用。55只SD大鼠随机分为正常对照组、流感病毒组、3 min组（高、中、低浓度）、6 min组（高、中、低浓度）、12 min组（高、中、低浓度）。3、6、12 min组大鼠给予相应浓度的银翘散溶液灌胃

正常对照组及流感病毒组给予等量生理盐水

每天2次

每次2 ml

共5天

腹主动脉取血制备含药血清。用甲型流感病毒鼠肺适应株FM1刺激小鼠巨噬细胞株Ana-1

同时给予不同煎煮时间下的银翘散含药血清进行干预

培养24 h后吸取上清

检测Toll样受体3、4型（TLR3、TLR4）mRNA和蛋白表达

及其下游髓样分化因子（My D88）依赖和非依赖途径相关因子（My D88、TRAF-6、TRIF、TRAM）mRNA的表达。结果与正常对照组比较

流感病毒组TLR3、TLR4、My D88、TRAF-6、TRAM、TRIF mRNA的表达水平均明显升高（P

0.05）;经不同煎煮时间、不同浓度银翘散干预后

各治疗组细胞的TLR3、TLR4、My D88、TRAF-6、TRAM和TRIF mRNA均明显下降（P

0.05）

尤以3 min组中、高浓度下降最为明显

较12 min组同浓度差异有统计学意义（P

0.05）。与流感病毒组比较

6 min组中浓度组对TLR3蛋白的表达有显著抑制作用（P

0.05）

3 min组和6 min组各浓度均可降低TLR4蛋白含量（P

0.05）。与12 min组同浓度比较

3 min组高浓度和6 min组中、高浓度对TLR4蛋白的表达有显著抑制作用（P

0.05）。结论银翘散抗甲型流感病毒作用的最佳煎煮时间可能为煮沸后3

min。

Abstract

Objective To study best decocting time of Yinqiao San against influenza A virus. Methods The researchers prepared powder of Yinqiao San respectively into solutions with concentration 0. 25 g / ml（ low）

0. 50 g /ml（ medium） and 1. 00 g / ml（ high）. Timing after those solutions were heated to 100℃

the three solutions were respectively boiled for 3

12 min and reposited in 4℃ to standby. Fifty-five SD rats were randomly divided into normal control group

influenza virus group

3 min group（ high

medium and low concentration）

6 min group（ high

medium and low concentration）

12 min group（ high

medium and low concentration）. The 3

12 min groups were given solutions of Yinqiao San with the corresponding concentration to gavage. The normal control group and the influenza virus group were given equal normal saline

2ml each time

twice a day for five days. Took blood from abdominal aorta to prepare medicated serum. Rat-lung adapted strains of influenza A virus（ FM1） was used to stimulate macrophage strains（ Ana-1） of rats under intervention with medicated serum with Yinqiao San with different decocting time and absorbed supernatant after those were cultivated for 24 h. Expression of toll-like receptors type 3

4（ TLR3

TLR4） mRNA and protein and mRNA of factors（ My D88

TRAF-6

TRIF

TRAM） related with dependent and independent pathway of its downstream myeloid differentiation factor（ My D88） were determined. Results Compared with the normal control group

expression levels of TLR3

TLR4

My D88

TRAF-6

TRAM

TRIF mRNA in the influenza virus group significantly increased（ P

0. 05）. Under intervention with Yinqiao San with different decocting time and concentration

TLR3

TLR4

My D88

TRAF-6

TRAM and TRIF mRNA of cells in each treatment group significantly decreased（ P

0. 05）

especially apparent in the 3 min group of medium and high concentration.There was statistically significant difference from the 12 min group with the same concentration（ P

0. 05）. Compared with the influenza virus group

the 6 min group with medium concentration significantly inhibite the expression of TLR3 protein（ P

0. 05）. In the 3 min group and the 6 min group with each concentration the content of TLR4 protein was reduced（ P

0. 05）. Compared with the 12 min group with the same concentration

the 3 min group with high concentration and the 6 min group with medium and high concentration had significant inhibition to expression of TLR4 protein（ P

0. 05）. Conclusion Best decoction time of Yinqiao San against influenza A virus was probably3

6 min after boiling.

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