Objective To research the prohibitive effect of steamed

water boiled and alcohol fried Huang Qin（ Radix Scutellariae） on influenza A virus subtype H1N1. Methods Randomly divided 120 ICR mice into normal control group（ n = 10）

model control group（ n = 10）

Ribavirin group（ n = 10）

steamed Huang Qin group（ n =30）

water boiled Huang Qin group（ n = 30） and alcohol fried Huang Qin group（ n = 30）. The steamed Huang Qin group

water boiled Huang Qin group and alcohol fried Huang Qin group were also respectively divided into high

middle and low dose subgroups

with 10 mice each. Mice in each group was infected by nasal dropping with 15 × LD50 A / PR /8 /34 plants of influenza virus bulk

except the normal control group

35 μl per mouse. The normal control group was nasal dripped with equivalent sterile saline. The Ribavirin group was given Ribavirin particle 82. 5 mg /（ kg·d） for gavage the day of being infected

once per day. The high

middle and low dose of the steamed Huang Qin group

the water boiled Huang Qin group and the alcohol fried Huang Qin group were given corresponding medicine 50

20

and 10 mg /（ kg·d） for gavage every day respectively

once per day. The normal control group and the model control group was given equivalent stilled water for gavage. All groups were gavaged for five days. Examined lung indexes and virus load of lung tissue of mice in each group. Calculated inhibition rate of lung indexes and virus.Results The lung indexes of mice in the model control group remarkably increased compared with the normal control group（ P < 0. 01）. The lung indexes of mice in the Ribavirin group

middle and high dose of the steamed Huang Qin group

the water boiled Huang Qin group and the alcohol fried Huang Qin group remarkably decreased compared with the model control group（ P < 0. 05 or P < 0. 01）. Inhibition rate of lung indexes in the Ribavirin group and in high dose of the alcohol fried Huang Qin group were respectively 62. 30% and 47. 54%

and the difference had no statistical significance（ P > 0. 05）. Inhibition rate of lung indexes in the steamed Huang Qin group

the water boiled Huang Qin group and the alcohol fried Huang Qin group increases as dose increases. Compared with the model control group

the Ribavirin group and each dose in the alcohol fried Huang Qin group could all decrease virus load of lung tissue of mice（ P < 0. 05 or P < 0. 01）. The difference in virus inhibition rates of different doses in the alcohol fried Huang Qin group had statistical significance（ P < 0. 05）

virus inhibition rate in the high dose group is highest

then in the middle dose group

and the lowest is in the low dose group. Conclusion Steamed Huang Qin

water boiled Huang Qin and alcohol fried Huang Qin all have various levels of antiviral function. The effect increases as dose increases. The antiviral function of alcohol fried Huang Qin was superior to that of the steamed Huang Qin and water boiled Huang Qin.