Objective To explore the possible mechanism of Yangxin Tongmai Decoction（养心通脉方） in the treatment of coronary heart disease. Methods Four rabbits were randomized into the serum containing medicine[8 ml /（ kg·d） of Yangxin Tongmai Decoction]group and the blank serum group（ equivoluminal normal saline）.Both groups were given intragastric administration for 5 days. After 0. 5 hour of the last administration

blood in common carotid artery was sampled to prepare serum containing Yangxin Tongmai Decoction and blank serum. Impact of serum containing medicine with different doses（ 1. 25%

2. 5%

5%

or 10%） and different intervening time（ 12 h

24 h

48 h

72 h

or 96 h） on the mitochondrial activity of rabbits’ bone marrow endothelial progenitor cells（ EPCs） was examined. The optimal intervening dose and time of serum containing Yangxin Tongmai Decoction was tested. After the cell density was regulated to 5 × 105 cells / ml

EPCs were divided into the treatment group and the control group. The two groups were cultured with the optimal dose of serum containing Yangxin Tongmai Decoction and blank serum respectively. After inventing for the optimal time

the multiplication

migration and adhesion ability of EPCs in both groups were examined. Results Compared with the control group at the same concentration

serum containing medicine at the concentration of 2. 5%

5% and 10% could enhance the mitochondrial activity of EPCs.With the increasing concentration

the mitochondrial activity was enhanced in the treatment group（ P < 0. 05）. Thus10% was the best intervening concentration. Compared with the control group at the same time point

the mitochondrial activity of EPCs was enhanced in the treatment group at 48 h

72 h and 96 h. Compared the treatment group at previous time points

the mitochondria of EPCs was more active at 72 h than that at 48 h

and more active at 96 h than that at 72 h（ P < 0. 05）. Thus the best intervening time was 96 h. The two groups were cultured with serum containing10% of Yangxin Tongmai Decoction and 10% blank serum for 96 h

respectively. The multiplication

migration and adhesion ability of EPCs in the treatment group was superior to that in the control group（ P < 0. 05）. Conclusion Serum containing Yangxin Tongmai Decoction might enhance the multiplication

migration and adhesion ability of EPCs. Then ischemic myocardial blood vessel angiogenesis might be promoted. This might be one of the mechanisms of Yangxin Tongmai Decoction in the treatment of coronary heart disease.