Objective To observe the effect of Biejia Jian Wan（鳖甲煎丸） medicine-containing serum of different concentration on angiogenesis. Methods Randomly divided 14 SD rats into the Biejia Jian Wan group（12 rats）and the saline group（2 rats）. The Biejia Jian Wan group was given Biejia Jian Wan 6 mg /（ kg·d） for gavage while the saline group was given the same volume of saline for gavage

both groups for 5 days. Took blood from abdominal aorta and separated serum one hour after the last gavage. Adopted in vitro cultured rat abdominal aorta ring to develop the model. Used culture medium contending Biejia Jian Wan serum of different concentrations（ containing 5%

10%

15%

20%

30% and 40% respectively） for culture. Set the control group（ serum of the saline group）

cultivated for 10 days in total. Observed and filmed microvascular growth images under inverted microscope. Calculated microvascular budding areas; Conducted immunohistochemical staining on the culture. Calculated the average density of microvascular in every high power field. Conducted Spearman ranking correlation analysis between density of nascent capillaries in aorta ring and the concentration of Biejia Jian Wan serum. Results Compared with the control group

microvascular budding areas of the 20%

15%

10% and 5% Biejia Jian Wan serum groups all remarkably decreased（ P < 0. 01）. There is no statistical significance in difference between microvascular budding areas of the30% and 40% Biejia Jian Wan serum groups（ P > 0. 05）. Microvascular budding area of the 15% Biejia Jian Wan serum group was the lowest

remarkably lower than the 5%

20%

30%

and 40% Biejia Jian Wan serum group（P < 0. 01）. But the difference between it and the 10% Biejia Jian Wan serum group has no statistical significance（P > 0. 05）. As is shown by related analysis results

when the concentration of Biejia Jian Wan serum≤15%

its function to inhibit angiogenesis in aorta ring generally reinforced and density of nascent capillaries in aorta ring decreased with concentration increased（ r =- 0. 637

P < 0.. 05） Conclusion Biejia Jian Wan had restraining function on new vessels and drug concentration of 15% seems to be the most effective.