Objective To investigate the possible mechanism of Bushen Jianpi Fang（ 补肾健脾方） in the treatment of ovarian reserve function decline. Methods Sixty mice were randomly divided into blank group

model group

premarin group and Bushen Jianpi Fang high

middle and low dose groups

with 10 mice in each group.Except for blank group

the other groups were established into mice model with immune ovarian function decline.Experiment began one week after the modeling. Bushen Jianpi Fang high

middle and low dose groups were given0. 54 g / ml

1. 08 g / ml

or 2. 16 g / ml of Bushen Jianpi Fang for gavage respectively

0. 3 ml each time. Premarin group was given 0. 3 ml of premarin tablet solution（ concentration of 0. 002 mg / ml） for gavage

while the blank group and model group were given 0. 3 ml of normal saline for gavage

respectively. The gavage lasted for 90 days

with one day off every week. Serum levels of follicle stimulating prime（ FSH）

luteinizing hormone（ LH） and estradiol（ E

2

）were tested

weight of ovary was measured

and ovarian tissues were stained with HE staining to count the number of oocytes and observe the ovarian morphology change under the electron microscope. Results Compared with those in the normal group mice

serum levels of FSH and LH were significantly increased

level of E

2

was significantly decreased

the ovarian weight was decreased

and the number of oocytes was decreased in the model group（ P

<

0. 01）. Compared with those in the model group

serum levels of LH and FSH reduced

level of E

2

weight of ovary and the number of oocytes increased in both the Bushen Jianpi Fang high dose group and premarin group（ P

<

0. 01）. Electron microscopy showed that the thickness of the oocyte zona pellucida of mice is not uniform

the boundary is not clear

with the myeloid bodies and many microvilli in the model group

but the thickness of the oocyte zona pellucida was more uniform

and the boundary was clear in the Bushen Jianpi Fang high dose group and the premarin group. Conclusion Bushen Jianpi Fang can regulate the level of sex hormone in mice with ovarian reserve function decline

and improve the quantity and quality of oocyte

which may be the one of the mechanisms of this herbal medicine in the treatment of ovarian reserve function decline.