Objective To observe the possible functional mechanism of Qingre Huoxue Fang（ 清热活血方） in treating rheumatoid arthritis in active phase. Methods Synovial tissues of patients with rheumatoid arthritis were separated to prepare primary generation of rheumatoid arthritis fibroblast-like synoviocytes（ RA-FLS）. Tumor necrosis factor α（ TNF-α） was adopted to induce migration and adhesion of RA-FLS. A blank group（ without medicine and TNF-α intervention）

an induced group（ induced by TNF-α of 10 or 20 ng / m L）

and the Qingre Huoxue Fang low

middle and high dose groups（ Qingre Huoxue Fang of 20

100

500 ng / m L respectively and induced by TNF-α） were set. Migration distance and square

OD value of adhesion and adherence inhibition rate of cells in each group were examined. And the expression of related proteins in PI3 K / Akt signal pathway

including PI3 K

p-Akt and Akt

was tested. Results Migration distance and square

adhesion OD value

as well as the expression of PI3 K and p-Akt protein increased in the induced group compared with those in the blank group（ P < 0. 01）; Compared with the induced group

each Qingre Huoxue Fang dose group could all restrain cell migration and adhesion

as well as the expression of PI3 K and p-Akt protein. It had the best effects when the dose was of 500 ng / m L（ P < 0. 05 or P <0. 01）. Conclusion By lowering the expression of protein PI3 K and p-Akt in RA-FLS

Qingre Huoxue Fang could relieve synovial inflammation and destruction of joint; thereby restrain migration and adhesion of RA-FLS.