Objective To explore the possible mechanism of Huayu Qutan Decoction（ 化瘀祛痰方） treating hyperlipemia. Methods Thirty rattus norregicus（ SD） rats were randomized into the blank control group

the hyperlipemia group and the Huayu Qutan group

10 rats in each group. After hyperlipemia models were established by feeding high fat diet

the Huayu Qutan group was given Huayu Qutan Decoction solution 10 ml /（ kg·d）. The blank control group and the hyperlipemia group were given equivoluminal phosphate buffered saline by gavage. After 30 days

rats’ serum total cholesterol（ TC）

triglyceride（ TG）

low density lipoprotein cholesterol（ LDL-C）

and high density lipoprotein cholesterol（ HDL-C） were detected. Liver morphological changes and hepatic lipid deposition were examined by hematoxylin and eosin（ HE） stain and oil red O stain. Related differential genes of rats’ hepatic autophagy bodies forming signal pathways were screened by polymerase chain reaction（ PCR） array. Realtime PCR was applied to detect the relating genes expressions in ubiquitin-like combination pathways containing Atg12-Atg5 pathway and LC3-PE pathway. Results There was lipid deposition in liver cells of hyperlipemia rats. Fat vacuole could be found in endochylema. Rats’ liver lipid droplet was reduced in the Huayu Qutan group. Compared with the blank control group

the levels of serum TC and LDL-C in the hyperlipemia group increased significantly

while the level of HDL-C decreased（ P < 0. 01）. Compared with the hyperlipemia group

the levels of serum TC

LDL-C and HDL-C in the Huayu Qutan group were all improved（ P < 0. 05 or P < 0. 01）. PCR array showed that 10 genes in the genes which participated in the formation of autophagy bodies changed differently by comparison among the three groups.Compared with the blank control group

the expression levels of Atg16l1、Atg3、Atg4c、Atg5 gene mRNA in the hyperlipemia group decreased（ P < 0. 05 or P < 0. 01）. Compared with the hyperlipemia group

the expression levels of Atg16l1、Atg3、Atg4c gene mRNA in the Huayu Qutan group increased（ P < 0. 05）. Conclusion Huayu Qutan Decoction might improve hyperlipemia rats’ liver lipid damage by regulating hyperlipemia rats’ mRNA expressions of Atg16l1

Atg3

Atg4 c and Atg5

which were related genes of hepatic autophagy bodies forming signal pathways.