Objective To evaluate two modeling methods of animal model of bronchial asthma with disease-syndrome combination of kidney-yang deficiency syndrome. Methods Fifty-four Wistar rats were divided into a blank group

an asthma group

a hormone group

a hormone + asthma group

an operation group

and an operation + asthma group

with 9 rats in each. Rats in the hormone groupand the hormone + asthma group were given 15 mg /（ kg·d）of hydrocortisone by subcutaneous injection

while the operation group and the operation + asthma group received bilateral total adrenalectomy to establish the kidney-yang deficiency syndrome model. The asthma group

the hormone +asthma group

and the operation + asthma group were given ovalbumin（ OVA） sensitization to induce asthma. General state

behavior

lung function and leukocyte classification in bronchoalveolar lavage fluid in each group were observed. At the same time

indexes of the function of hypothalamic-pituitary-adrenal axis in serum [including corticotropin-releasing hormone（ CRH）

adrenocorticotropic hormone（ ACTH）

cortisol（ CORT）

interferon-γ（ INF-γ）

immune globulin E（ Ig E） ]

and levels of each inflammatory factor in lung tissue were examined

and pathologic changes in the lung tissue were observed. Results In the hormone + asthma group and the operation + asthma group

total number of leukocyte and eosinophils in bronchoalveolar lavage fluid increased

while the excretion of CRH

ACTH and CORT decreased; levels of total serum Ig E and OVA-specific Ig E increased; expression and excretion of INF-γ in lung tissues decreased while the expression and excretion of interleukin-4（ IL-4） increased（ P <0. 05 or P < 0. 01）; There were large amounts of inflammation infiltrations and mucosa edema of air passage in bronchus. There was no significant difference in inflammatory cells in bronchoalveolar lavage fluid when comparing the hormone + asthma group and the operation + asthma group. Indexes of HPA axis function in the operation + asthma group decreased more remarkably while pathologic changes in lung tissue in the hormone + asthma group were more remarkable. Conclusion Both two animal models of bronchial asthma with kidney-yang deficiency syndrome could reflect clinical characters of bronchial asthma of kidney-yang deficiency syndrome. But animal model by bilateral total adrenalectomy seems to have more remarkable influence on HPA axis function.