Impact of Serum Containing Xiaoyao Pill （逍遥丸） on Smads Expression in Oocyte of Mice’s Preantral Follicle Growth in Vitro

SUN Xiaohuan; LIU Yahua; WEI Xuecong; LI Li; FAN Lijie; GU Xiangxiang; GENG Dandan; DU Huilan

doi:10.13288/j.11-2166/r.2016.23.015

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Impact of Serum Containing Xiaoyao Pill （逍遥丸） on Smads Expression in Oocyte of Mice’s Preantral Follicle Growth in Vitro

更新时间：2021-11-17

- Impact of Serum Containing Xiaoyao Pill （逍遥丸） on Smads Expression in Oocyte of Mice’s Preantral Follicle Growth in Vitro
- Journal of Traditional Chinese Medicine Vol. 57, Issue 23, Pages: 2037-2041(2016)
- 作者机构：
  
  河北医科大学
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2016.23.015
  CLC： R285.5
- Published：2016
- 稿件说明：
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[1]孙晓换,刘亚华,魏学聪,李丽,范丽洁,谷向向,耿丹丹,杜惠兰.逍遥丸含药血清对体外生长小鼠腔前卵泡卵母细胞中Smads表达的影响[J].中医杂志,2016,57(23):2037-2041.

SUN Xiaohuan, LIU Yahua, WEI Xuecong, et al. Impact of Serum Containing Xiaoyao Pill （逍遥丸） on Smads Expression in Oocyte of Mice’s Preantral Follicle Growth in Vitro[J]. Journal of Traditional Chinese Medicine2016, 57(23): 2037-2041.
[1]孙晓换,刘亚华,魏学聪,李丽,范丽洁,谷向向,耿丹丹,杜惠兰.逍遥丸含药血清对体外生长小鼠腔前卵泡卵母细胞中Smads表达的影响[J].中医杂志,2016,57(23):2037-2041. DOI： 10.13288/j.11-2166/r.2016.23.015.

SUN Xiaohuan, LIU Yahua, WEI Xuecong, et al. Impact of Serum Containing Xiaoyao Pill （逍遥丸） on Smads Expression in Oocyte of Mice’s Preantral Follicle Growth in Vitro[J]. Journal of Traditional Chinese Medicine2016, 57(23): 2037-2041. DOI： 10.13288/j.11-2166/r.2016.23.015.

摘要

目的探讨逍遥丸提高体外卵母细胞质量的可能作用机制。方法分离58只昆明小鼠腔前卵泡

分别以10%正常大鼠血清

10%逍遥丸低、中、高剂量大鼠含药血清

10%逍遥丸高剂量大鼠含药血清+10μmol/L K02288孵育

设为对照组

疏肝低、中、高剂量组

抑制剂组

培养6天。实时荧光定量PCR法检测卵母细胞中Smad1、Smad5、Smad8 mRNA表达

细胞免疫荧光检测Smad1、Smad5、Smad8和磷酸化的Smad1/5/8（p-Smad1/5/8）蛋白表达。结果疏肝高剂量组Smad1 mRNA和蛋白高于对照组（P<0.05）;疏肝各剂量组Smad5、Smad8 mRNA和蛋白及p-Smad1/5/8蛋白均高于对照组（P<0.05）

其中疏肝高、中剂量组Smad5 mRNA和蛋白比较差异无统计学意义（P>0.05）

均高于低剂量组（P<0.05）

Smad8 mRNA和蛋白及p-Smad1/5/8蛋白以疏肝高剂量组最高（P<0.05）;抑制剂组p-Smad1/5/8蛋白低于疏肝高剂量组（P<0.05）。结论逍遥丸提高体外卵母细胞质量的作用可能与上调卵母细胞中Smad1、Smad5、Smad8 mRNA和蛋白及p-Smad1/5/8蛋白表达有关

以高剂量组效果更佳。

Abstract

Objective To explore the possible mechanism of Xiaoyao Pill in improving the quality of oocyte in vitro. Methods Preantral follicles of 56 Kunming mice were separated and divided into the control group

the dispersing the stagnated liver-energy low

medium and high dose groups and the inhibitor group. Preantral follicles in each group were incubated respectively with 10% serum of normal rats

10% rats’ serum containing low

medium and high dose Xiaoyao Pill

as well as 10% rats’ serum containing high dose Xiaoyao Pill + 10 μmol / L K02288

all for6 days. The expressions of Smad1

Smad5 and Smad8 mRNA in oocyte were detected by real-time fluorescent quantitative polymerase chain reaction（ PCR） method. The expressions of Smad1

Smad5

Smad8

as well as phosphorylated Smad1 /5 /8（ p-Smad1 /5 /8） protein were detected by cellular immunofluorescence. Results Expressions of Smad1 mRNA and protein in the dispersing the stagnated liver-energy high dose group were higher than those in the control group（ P < 0. 05）. Expression of Smad5

Smad8 mRNA

protein and p-Smad1 /5 /8 protein in all dispersing the stagnated liver-energy groups were higher than those in the control group（ P < 0. 05）. There was no significant difference in expressions of Smad5 mRNA and protein between the dispersing the stagnated liver-energy high dose group and medium dose group（ P > 0. 05）

which were both higher than those in the low dose group（ P < 0. 05）.Expressions of Smad8 mRNA

protein and p-Smad1 /5 /8 protein were the highest in the dispersing the stagnated liverenergy high dose group（ P < 0. 05）. P-Smad1 /5 /8 protein in the inhibitor group was lower than that in the dispersing the stagnated liver-energy high dose group（ P < 0. 05）. Conclusion Xiaoyao Pill improving the quality of oocyte in vitro might be relate to up-regulating the expressions of Smad1

Smad5

Smad8 mRNA

protein and p-Smad1 /5 /8protein. The effect in the high dose group is the best.

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