Effect of Qiling Granule（杞灵颗粒） on Oxidative Stress Injury of Retinal Pigment Epitheliu in Mice

AN Na; ZHUANG Zengyuan; LIANG Lina; CHEN Qiang; HOU Le

doi:10.13288/j.11-2166/r.2017.03.018

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Effect of Qiling Granule（杞灵颗粒） on Oxidative Stress Injury of Retinal Pigment Epitheliu in Mice

更新时间：2021-11-10

- Effect of Qiling Granule（杞灵颗粒） on Oxidative Stress Injury of Retinal Pigment Epitheliu in Mice
- Journal of Traditional Chinese Medicine Vol. 58, Issue 3, Pages: 250-254(2017)
- 作者机构：
  
  1. 中国中医科学院眼科医院
  2. 国家食品药品监督管理总局药品审评中心
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2017.03.018
  CLC： R285.5
- Published：2017
- 稿件说明：
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[1]安娜,庄曾渊,梁丽娜,陈强,侯乐.杞灵颗粒对小鼠视网膜色素上皮细胞氧化应激损伤的影响[J].中医杂志,2017,58(03):250-254.

AN Na, ZHUANG Zengyuan, LIANG Lina, et al. Effect of Qiling Granule（杞灵颗粒） on Oxidative Stress Injury of Retinal Pigment Epitheliu in Mice[J]. Journal of Traditional Chinese Medicine2017, 58(3): 250-254.
[1]安娜,庄曾渊,梁丽娜,陈强,侯乐.杞灵颗粒对小鼠视网膜色素上皮细胞氧化应激损伤的影响[J].中医杂志,2017,58(03):250-254. DOI： 10.13288/j.11-2166/r.2017.03.018.

AN Na, ZHUANG Zengyuan, LIANG Lina, et al. Effect of Qiling Granule（杞灵颗粒） on Oxidative Stress Injury of Retinal Pigment Epitheliu in Mice[J]. Journal of Traditional Chinese Medicine2017, 58(3): 250-254. DOI： 10.13288/j.11-2166/r.2017.03.018.

摘要

目的探讨杞灵颗粒治疗年龄相关性黄斑变性（AMD）的可能作用机制。方法小鼠予0.8%氢醌饮食饲养5个月建立小鼠AMD模型

随机分为模型组、杞灵低剂量组、杞灵中剂量组、杞灵高剂量组和叶黄素组（分别给予等体积蒸馏水

杞灵颗粒干浸膏3.75、7.5和15 g/kg

叶黄素片水溶液0.18 g/kg灌胃）

每组17只。予灌胃治疗

同时18只小鼠常规饲养作为正常组。观察各组小鼠视网膜病理形态

视网膜色素上皮细胞（RPE）-Bruch膜-脉络膜超微结构

RPE细胞凋亡率和8-羟基脱氧鸟苷（8-OHd G）的表达

以及血清总超氧化物歧化酶（T-SOD）、谷胱甘肽过氧化物酶（GSH-PX）含量。结果与模型组比较

治疗14天时

杞灵高、中、低剂量组和叶黄素组对RPE细胞数量有保护作用（P<0.05或P<0.01）;28天时

杞灵低、中剂量组和叶黄素组对RPE细胞数量有明显保护作用（P<0.01）。透射电子显微镜观察

模型组和叶黄素组RPE细胞内出现空泡样改变

玻璃膜（Bruch膜）比正常组厚

外胶原层见层状沉积物。杞灵中剂量组超微结构基本正常。TUNEL检测示

与模型组比较

14天时杞灵低、中剂量组和叶黄素组及28天时杞灵中剂量组和叶黄素组对RPE细胞凋亡有抑制作用（P<0.05或P<0.01）。8-OHd G免疫荧光法发现

与模型组比较

14天时

杞灵低、中剂量组和叶黄素组对RPE细胞DNA有显著保护作用（P<0.05或P<0.01）;28天时

杞灵低、中剂量组有保护作用（P<0.05或P<0.01）。造模后14天和28天

杞灵中剂量组T-SOD活性均高于模型组（P<0.05）

而在保护GSH-PX活性方面

仅28天时叶黄素组GSH-PX活性高于模型组（P<0.05）。结论杞灵颗粒可减轻AMD模型小鼠RPE-Bruch膜-脉络膜的损伤

降低RPE细胞的凋亡率

可能是其治疗AMD作用机制之一。

Abstract

Objective To explore the potential mechanism of Qiling Granule（ QL

杞灵颗粒） in the treatment of age-related macular degeneration（ AMD）. Methods The mice were given 0. 8% hydroquinone（ HQ） diet for 5months to build AMD model then were randomized into model group

low dose QL group

medium dose QL group

high dose QL group and lutein group（ respectively given the same volume of distilled water

Qiling Granule dry extract of 3. 75

7. 5 and 15 g / kg and Lutein Tablet solution of 0. 18 g / kg）

17 mice in each group. The drugs were administered by gavage

and another 18 mice were given conventional diet as control group. Then we observed the retinal pathological morphology of the mice

the ultrastructure of retinal pigment epithelium（ RPE）- Bruch membrane choroid

the apoptosis rate of RPE

the expression of 8-hydroxy-2-deoxyguanosine（ 8-OHd G） and the volume of serum total superoxide dismutase and glutathione peroxidase（ GSH-PX）. Results Compared with the model group

on the 14 th day of treatment

the low

medium dose QL group and the lutein group showed a protective action on RPE number（ P < 0. 05 or P < 0. 01）; on the 28 th day

they showed a significant protection action（ P < 0. 01）.Under transmission electron microscope

RPE in the model group and the lutein group showed vacuolization

the Bruch membranes were thicker than those in the control group and layered sediments could be seen in the outer collagenous zone. The ultrastructure in medium dose QL group was normal basically. Through TUNEL method

compared with the model group

on the 14 th day

the low dose

medium dose QL group and the lutein group and on the28 th day

the medium dose group and the lutein group showed suppression on the apoptosis of RPE（ P < 0. 05 or P <0. 01）. By 8-OHd G immunofluorescence method

compared with the model group

on the 14 th day

the low

medium dose QL group and the lutein group

and on the 28 th day

the low and medium dose QL group showed significant protective action on DNA of RPE（ P < 0. 05 or P < 0. 01）. On the 14 th and the 28 th day after modeling

the T-SOD activity in the medium dose group was higher than that in the model group（ P < 0. 05）; but for protecting GSH-PX

the GSH-PX activity in the lutein group was higher than that in the model group just on the 28 th day（ P < 0. 05）.Conclusion Qiling Granule could reduce the damage of RPE-Bruch membrane choroid and lower the apoptosis rate of RPE

which might be the mechanism in treating AMD.

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Related Institution

College of Pediatrics，Henan University of Chinese Medicine

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