Objective To discuss the potential mechanism of Huoxue Yiqi Fang（ 活血益气方） and its decomposed recipes in promoting angiogenesis. Methods The model of human umbilical vein endothelial cell（ HUVEC）in hypoxia was built and divided into the model group

Huoxue Yiqi Fang（ HYF） group

Huoxue Fang（ HF） group and Yiqi Fang（ YF） Group. There was also a control group set in conventional cultivation. The three Chinese medicine groups were cultivated by 10% serum containing corresponding herbs

while the model group and the control group were by serum containing normal saline. They were all intervened for 24 hours. The cell proliferation was detected by CCK-8 method

the HUVEC apoptosis was detected by Annexin V-FITC/PI double staining

and the super oxide dismutase（ SOD） activity was detected by WST-1 method

the malondialdehyde（ MDA） volume and the lactate dehydrogenase（ LDH） volume in cell culture supernatant were detected by microplate test

and the mRNA levels of protein kinase C（ PKC）

Ras and Raf-1 were detected by real-time quantitative PCR. Results Compared with the control group

in the model group

the HUVEC proliferation

the SOD activity and the PKC

Ras

Raf-1 mRNA declined significantly

and the apoptosis and MDA and LDH volume increased significantly（ P < 0. 05 or P < 0. 01）.Compared with the model group

in the HYF group

HF group and YF group

HUVEC proliferation increased significantly

LDH volume decreased significantly; SOD activity in HYF Group improved significantly; the apoptosis in HYF group and HF group descended significantly; the MDA volume in HYF group and YF group reduced significantly

while the RKC

Ras and Raf-1 mRNA volume ascended significantly（ P < 0. 05 or P < 0. 01）. Conclusion HYF and its decomposed recipes could promote the proliferation of HUVEC in hypoxia and inhibit its apoptosis in varying degrees. The mechanism may be enhancing the cellular antioxidant activity and regulating PKC/Ras/Raf-1 signaling pathway. HQF could protect cells better than HF and YF

so HF and YF coordinate together.