Objective To explore the potential anti-atherosclerosis mechanism of Huxin Fang（ 护心方

HF）.Methods THP-1 cells were incubated with 160nmol/L phorbol esters polyclonal stimulants for 48 hours to differentiate into macrophages

and then 50mg/L oxidized low-density lipoprotein（ ox-LDL） were added for another 48 hours to make them transfer into foam cells. After that

serum containing 40% HF were added for 12 hours. Autophagyassociated proteins microtube-associated protein light chain 3Ⅱ（ LC3-Ⅱ）

microtubule-associated protein light chain3Ⅰ（ LC3-Ⅰ）

Beclin and p62 expressions were detected by Western blotting

while the distribution and content of autophagic protein index LC3 B were observed by laser confocal microscopy. Results Compared with the blank group

the expression of LC3-Ⅱ/LC3-Ⅰ and Beclin-1 in the model group significantly increased

the expression of p62 decreased and the average fluorescence intensity of LC3 B significantly increased（ P < 0. 05）. Compared with the model group

the expression of LC3-Ⅱ/LC3-Ⅰ and Beclin-1 in the HF group significantly decreased

the expression of p62 significantly increased and the average fluorescence intensity of LC3 B decreased（ P < 0. 05）. Conclusion HF could reverse the expression of the autophagy-related proteins induced by ox-LDL

thereby resisting excessive autophagy during arteriosclerosis. This might be one of the possible anti-atherosclerosis mechanism of HF.