Objective To explore the molecular biological characteristics of spleen-qi deficiency syndrome.Methods Twelve Sprague Dawley rats were randomized into a normal group and a spleen-qi deficiency group（n = 6 in each）. Rats in the spleen-qi deficiency group were made spleen-qi deficiency models by improper diet and exhaustive swimming. Ileal total protein was separated and extracted. The differential expression proteins and the expression ratio in both groups were detected and analyzed with isobaric tags for relative and absolute quantitation（i TRAQ）method. The expressions of differential expression proteins

such as small intestinal fatty acid binding protein（IFABP）

liver fatty acid binding protein（L-FABP） and Niemann-Pick type C1 Like 1（NPC1 L1）

were verified by Western blot method. Results i TRAQ technology analysis showed that there were difference in fatty digestion and absorption related pathway proteins in rats between the two groups. Compared with those in the normal group

IFABP

microsomal triglyceride transfer protein（MTTP）

L-FABP

NPC1 L1

apolipoprotein B（Apo B）

apolipoprotein A4（Apo A4） and monoacylglycerol acyl transferase（Mogat 2） in the spleen-qi deficiency group were upregulated（fold change more than 1. 2）. It was verified by Western blot method that the change trend of differential proteins I-FABP

L-FABP and NPC1 L1 was in accord with the result of proteome. Conclusion The expressions of I-FABP

MTTP

L-FABP

NPC1 L1

Apo B

Apo A4 and MGAT7 proteins in ileal tissue of rats with spleen-qi deficiency syndrome are up-regulated.