Effect of Zhibai Dihuang Decoction （ 知柏地黄汤） on Energy Metabolism of Sperm Mitochondria in Rats with Ureaplasma Urealyticum Infection

BIN Donghua; WANG Sunya; HE Qinghu

doi:10.13288/j.11-2166/r.2018.03.013

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Effect of Zhibai Dihuang Decoction （知柏地黄汤） on Energy Metabolism of Sperm Mitochondria in Rats with Ureaplasma Urealyticum Infection

更新时间：2021-11-10

- Effect of Zhibai Dihuang Decoction （知柏地黄汤） on Energy Metabolism of Sperm Mitochondria in Rats with Ureaplasma Urealyticum Infection
- Journal of Traditional Chinese Medicine Vol. 59, Issue 3, Pages: 231-234(2018)
- 作者机构：
  
  1. 湖南中医药大学第一附属医院
  2. 湖南中医药大学
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2018.03.013
  CLC： R-332;R285.5
- Published：2018
- 稿件说明：
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[1]宾东华,王孙亚,何清湖.知柏地黄汤对解脲脲原体感染模型大鼠精子线粒体能量代谢的影响[J].中医杂志,2018,59(03):231-234.

BIN Donghua, WANG Sunya, HE Qinghu. Effect of Zhibai Dihuang Decoction （知柏地黄汤） on Energy Metabolism of Sperm Mitochondria in Rats with Ureaplasma Urealyticum Infection[J]. Journal of Traditional Chinese Medicine2018, 59(3): 231-234.
[1]宾东华,王孙亚,何清湖.知柏地黄汤对解脲脲原体感染模型大鼠精子线粒体能量代谢的影响[J].中医杂志,2018,59(03):231-234. DOI： 10.13288/j.11-2166/r.2018.03.013.

BIN Donghua, WANG Sunya, HE Qinghu. Effect of Zhibai Dihuang Decoction （知柏地黄汤） on Energy Metabolism of Sperm Mitochondria in Rats with Ureaplasma Urealyticum Infection[J]. Journal of Traditional Chinese Medicine2018, 59(3): 231-234. DOI： 10.13288/j.11-2166/r.2018.03.013.

摘要

目的探讨知柏地黄汤治疗解脲脲原体感染所致男性不育症的可能机制。方法采用经大鼠膀胱注射解脲脲原体标准株菌液2 ml/只建立解脲脲原体感染大鼠模型

将造模成功的64只大鼠随机分为中药组、西药组、中西组、模型组

每组16只

另设假手术组15只。造模成功后第2天假手术组、模型组用0.9%氯化钠注射液2 ml/d灌胃

中药组给予知柏地黄汤1.88 g生药/d

西药组给予强力霉素4 mg/d

中西组给予等量的知柏地黄汤与强力霉素

各组均连续灌胃21天。检测大鼠精子线粒体膜通道孔（m PTP）开放程度及精子线粒体二磷酸腺苷（ADP）、三磷酸腺苷（ATP）、一磷酸腺苷（AMP）含量

计算能荷（EC）值。结果与假手术组比较

模型组大鼠精子m PTP开放吸光度值下降

精子ATP、ADP及EC降低

AMP值上升（P<0.01）。与模型组比较

各治疗组大鼠精子m PTP开放吸光度值均有上升

西药组、中西组大鼠精子ATP、ADP及EC值上升

AMP值下降（P<0.05或P<0.01）。与中药组比较

中西组大鼠精子m PTP开放吸光度值上升（P<0.05）;中西组大鼠精子ATP高于中药组和西药组

中西组ADP及EC值高于中药组（P<0.05或P<0.01）。结论知柏地黄汤能够降低解脲脲原体感染大鼠精子m PTP开放程度

维护线粒体能量代谢

进而提高精子质量

这可能是其治疗解脲脲原体感染所致不育症的机制之一。

Abstract

Objective To explore the potential mechanism of Zhibai Dihuang Decoction（知柏地黄汤

ZDD） in treating male infertility caused by ureaplasma urealyticum infection. Methods Ureaplasma urealyticum standard strain liquid（ 2 ml per rat） was injected through bladder to establish rat model of ureaplasma urealyticum infection.The 64 successfully modelled rats were randomized into Chinese medicine group

western medicine group

the integrative medicine group and the model group

with 16 rats in each group

and another 15 rats were sat in the sham operation group. On the second day after model establishment

the sham operation group and the model group were orally administered 0. 9% sodium chloride injection（ 2 ml/d）

Chinese medicine group received crude drug of ZDD（ 1. 88 g/d）

Western medicine group received 4 mg/d of doxycycline

and the integrative medicine group was given equal amount of ZDD and doxycycline. Each group was treated for 21 days. The open degree of mitochondrial membrane pore（ m PTP） and the content of mitochondrial ATP

ATP and AMP in the sperm were measured and the energy charge（ EC） was calculated. Results Compared with those in the sham operation group

the open light absorbance of sperm m PTP in the model group decreased

ATP

ADP and EC decreased

while AMP increased（ P <0. 01）. Compared with the model group

the open light absorbance of sperm m PTP increased in all treatment groups

and ATP

ADP and EC in Western medicine group and the integrative medicine group increased while the AMP decreased（ P < 0. 05 or P < 0. 01）. Compared with Chinese medicine group

the open light absorbance of sperm m PTP in the integrative medicine group increased（ P < 0. 05）; ATP in the integrative medicine group was higher than that in Chinese medicine group and Western medicine group

and the ADP and EC in the integrative medicine group were higher than those in Chinese medicine group（ P < 0. 05 or P < 0. 01）. Conclusion ZDD could reduce the open degree of sperm m PTP in rats infected with ureaplasma urealyticum and maintain the energy metabolism of mitochondria

thereby improving the sperm quality

which may be one of the mechanisms for its treatment of infertility caused by ureaplasma urealyticum infection.

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