Effect of Bushen Shuji Granules（补肾舒脊颗粒） Drug Containing Serum on mRNA and Protein Expressions of Wnt5a and β-catenin in Human Bone Marrow Mesenchymal Stem Cells

YAN Zeran; SHI Guangyao; WANG Xiaotong; ZHANG Lan; YAN Xiaoping

doi:10.13288/j.11-2166/r.2018.05.014

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Effect of Bushen Shuji Granules（补肾舒脊颗粒） Drug Containing Serum on mRNA and Protein Expressions of Wnt5a and β-catenin in Human Bone Marrow Mesenchymal Stem Cells

更新时间：2021-11-10

- Effect of Bushen Shuji Granules（补肾舒脊颗粒） Drug Containing Serum on mRNA and Protein Expressions of Wnt5a and β-catenin in Human Bone Marrow Mesenchymal Stem Cells
- Journal of Traditional Chinese Medicine Vol. 59, Issue 5, Pages: 415-419(2018)
- 作者机构：
  
  1. 中日友好医院
  2. 内蒙古医科大学
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2018.05.014
  CLC： R285
- Published：2018
- 稿件说明：
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[1]鄢泽然,史光耀,王晓曈,张岚,阎小萍.补肾舒脊颗粒含药血清对人骨髓间充质干细胞Wnt5a、β-catenin mRNA及蛋白表达的影响[J].中医杂志,2018,59(05):415-419.

YAN Zeran, SHI Guangyao, WANG Xiaotong, et al. Effect of Bushen Shuji Granules（补肾舒脊颗粒） Drug Containing Serum on mRNA and Protein Expressions of Wnt5a and β-catenin in Human Bone Marrow Mesenchymal Stem Cells[J]. Journal of Traditional Chinese Medicine2018, 59(5): 415-419.
[1]鄢泽然,史光耀,王晓曈,张岚,阎小萍.补肾舒脊颗粒含药血清对人骨髓间充质干细胞Wnt5a、β-catenin mRNA及蛋白表达的影响[J].中医杂志,2018,59(05):415-419. DOI： 10.13288/j.11-2166/r.2018.05.014.

YAN Zeran, SHI Guangyao, WANG Xiaotong, et al. Effect of Bushen Shuji Granules（补肾舒脊颗粒） Drug Containing Serum on mRNA and Protein Expressions of Wnt5a and β-catenin in Human Bone Marrow Mesenchymal Stem Cells[J]. Journal of Traditional Chinese Medicine2018, 59(5): 415-419. DOI： 10.13288/j.11-2166/r.2018.05.014.

摘要

目的探讨补肾舒脊颗粒延缓异位骨化的可能作用机制。方法体外培养人骨髓间充质干细胞（BMSCs）分为空白组、模型组、对照组及中药高、中、低剂量组。空白组加入基础培养基（3.8 ml）+5%正常大鼠血清（0.2 ml）;模型组加入诱导培养基（4.8 ml）+5%正常大鼠血清（0.2 ml）;西药组加入诱导培养基（3.8 ml）+5%洛索洛芬钠含药血清（0.2 ml）;中药高、中、低剂量组分别加入诱导培养基（3.8 ml）+5%补肾舒脊颗粒含药血清（0.2 ml）。28天后通过逆转录-聚合酶链反应（RT-PCR）和免疫印迹试验（Western blot）检测Wnt5a mRNA、β-catenin mRNA及蛋白表达。结果与空白组比较

模型组Wnt5a、β-catenin蛋白及mRNA表达均升高（P<0.05或P<0.01）;与模型组比较

对照组和中药各剂量组Wnt5a、β-catenin蛋白及mRNA表达均降低（P<0.05或P<0.01）;与对照组比较

中药高剂量组Wnt5a mRNA、β-catenin mRNA表达降低

中药中剂量组Wnt5a、β-catenin蛋白表达降低（P<0.05或P<0.01）;与中药低、中剂量组比较

中药高剂量组Wnt5a mRNA、β-catenin mRNA表达降低（P<0.05或P<0.01）。与中药低剂量组比较

中药中剂量组Wnt5a蛋白表达降低

中药高剂量组β-catenin蛋白表达升高（P<0.05）。结论补肾舒脊颗粒可能通过Wnt/β-catenin信号通路抑制Wnt5a mRNA、β-catenin mRNA及蛋白的表达

从而起到延缓强直性脊柱炎异位骨化的作用。

Abstract

Objective To explore the possible mechanism of Bushen Shuji Granules（补肾舒脊颗粒

BSG） in delaying heterotopic ossification. Methods Human bone marrow mesenchymal stem cells（BMSCs） were cultured in vitro and divided into blank group

model group

control group and Chinese medicine high

medium and low dose groups. The blank group was cultured with basal medium（3. 8 ml） + 5% normal rat serum（0. 2 ml）; the model group was cultured with induction medium（4. 8 ml） + 5% normal rat serum（0. 2 ml）; the control group was cultured with induction medium（3. 8 ml） + 5% loxoprofen sodium containing serum（0. 2 ml）; the Chinese medicine high

medium and low dose groups were cultured with induction medium（3. 8 ml） + 5% BSGs containing serum（0. 2 ml）. After 28 days

the mRNA and protein expressions of Wnt5 a and β-catenin were detected by reverse transcription-polymerase chain reaction（RT-PCR） and Western blotting. Results Compared with those in the blank group

Wnt5 a and β-catenin protein and mRNA expressions in the model group significantly increased（P < 0. 05 or P < 0. 01）. Compared with those in the model group

the mRNA and protein expressions of Wnt5 a and β-catenin in the control group and Chinese medicine high

medium and low dose groups decreased（P < 0. 05 or P < 0. 01）; compared with those in the control group

the mRNA expressions of Wnt5 a and β-catenin in Chinese medicine high dose group decreased

while the protein expressions of Wnt5 a and β-catenin in Chinese medicine medium dose group decreased（P < 0. 05 or P < 0. 01）; compared with those in Chinese medicine low and medium dose groups

the mRNA expressions of Wnt5 a and β-catenin in the high dose group decreased（P < 0. 05 or P < 0. 01）. Compared with those in Chinese medicine low dose group

the Wnt5 a protein expression in the medium-dose group decreased

and the β-catenin protein expression in the high-dose group increased（P < 0. 05）. Conclusion BSG may inhibit the mRNA and protein expressions of Wnt5 a and β-catenin through the Wnt/β-catenin signaling pathway

which can delay the heterotopic ossification of ankylosing spondylitis.

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Related Institution

Guang 'anmen Hospital，China Academy of Chinese Medical Sciences

Graduate School of Beijing University of Chinese Medicine

National Data Center of Traditional Chinese Medicine，China Academy of Chinese Medical Sciences

Beijing University of Chinese Medicine

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