Objective To explore the possible mechanism of Gouteng（Uncariae Ramulus Cum Uncis

钩藤） in treating hypertension of ascendant hyperactivity of liver Yang syndrome. Methods Fourteen SHR rats were randomized into ascendant hyperactivity of liver Yang syndrome（AHLY） group and Gouteng group

with 7 rats in each group. Another seven Wistar rats were selected as control group. AHLY group and Gouteng group were given oral Fuzi（Aconiti Lateralis Radix Praeparata

附子） solution of 20 ml/kg to build the hypertension rat model of AHLY syndrome. After successful modeling

Gouteng group was given 22. 9 ml/kg Gouteng decoction

while the AHLY group and the control group were respectively given the same amount of normal saline; the rats in each group were intervened for 4 weeks. Then the macro-indicators such as systolic blood pressure

facial temperature

and pain threshold and rotation time were collected. At the same time

the information of endogenous metabolites in serum of rats was collected by UHPLC-quadrupole-electrostatic field orbitrap high resolution mass（UPLC-Q-Exactive） technology. Partial least-squares regression was used to analyze the relationship between external macro-indicators and internal microindicators. Results Compared with those in the control group

the systolic blood pressure increased

the facial temperature increased

the pain threshold decreased and the rotation time shortened in the model group

while the systolic blood pressure in Guoteng group was lower than that in the AHLY group

and the rotation time was longer（P < 0. 05 or P < 0. 01）. A total of 16 biomarkers were identified by database search

including linoleic acid

dopamine

deoxycorticosterone

4-hydroxyproline

dihydrotestosterone

11 b-hydroxyprogesterone

glycylglycine

6-ketone prostaglandin

16 a-hydroxyandrostone

L-tryptophan

leukotriene B4

thromboxane B2

phosphatidic acid

2-methoxy estrone

uric acid

and lysophosphatidylcholine. Compared with those in the control group

the levels of dihydrotestosterone

11 b-hydroxyprogesterone

deoxycorticosterone

2-methoxy estrone decreased（P < 0. 05 or P <0. 01）

and the rest of the biomarkers increased in the AHLY group（P < 0. 05 or P < 0. 01）; compared with those in the AHLY group

the levels of dihydrotestosterone

16 a-hydroxyandrostaglandin

deoxycorticosterone and 2-methoxy estrone in Gouteng group increased（P < 0. 05 or P < 0. 01）

and the rest of the biomarkers decreased（P < 0. 05 or P < 0. 01）. Conclusion The possible mechanism of Gouteng in treating hypertension of the ascendant hyperactivity of liver Yang syndrome may include the following: regulating the metabolisms of glycine

serine and threonine

regulating the metabolisms of arachidonic acid and tyrosine to reduce facial temperature

regulating the metabolisms of glycerophospholipid and linoleic acid and the anabolism of sterol hormones to improve dizziness as well as regulating metabolisms of arachidonic acid

tyrosine and purine to improve the pain threshold.