Impact of Needling Point “Renying（ST9）” on Hypothalamic Gene Expression of Spontaneous Hypertension Rats

AN Na; GUO Yan; LU Juan; ZHAO Ruili; MA Tian; SHI Xuemin; GUO Changqing

doi:10.13288/j.11-2166/r.2018.06.017

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Impact of Needling Point “Renying（ST9）” on Hypothalamic Gene Expression of Spontaneous Hypertension Rats

更新时间：2021-11-10

- Impact of Needling Point “Renying（ST9）” on Hypothalamic Gene Expression of Spontaneous Hypertension Rats
- Journal of Traditional Chinese Medicine Vol. 59, Issue 6, Pages: 518-522(2018)
- 作者机构：
  
  1. 北京中医药大学
  2. 天津中医药大学第一附属医院
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2018.06.017
  CLC： R245
- Published：2018
- 稿件说明：
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[1]安娜,郭妍,芦娟,赵瑞利,马田,石学敏,郭长青.针刺“人迎”穴对自发性高血压大鼠下丘脑基因表达的影响[J].中医杂志,2018,59(06):518-522.

AN Na, GUO Yan, LU Juan, et al. Impact of Needling Point “Renying（ST9）” on Hypothalamic Gene Expression of Spontaneous Hypertension Rats[J]. Journal of Traditional Chinese Medicine2018, 59(6): 518-522.
[1]安娜,郭妍,芦娟,赵瑞利,马田,石学敏,郭长青.针刺“人迎”穴对自发性高血压大鼠下丘脑基因表达的影响[J].中医杂志,2018,59(06):518-522. DOI： 10.13288/j.11-2166/r.2018.06.017.

AN Na, GUO Yan, LU Juan, et al. Impact of Needling Point “Renying（ST9）” on Hypothalamic Gene Expression of Spontaneous Hypertension Rats[J]. Journal of Traditional Chinese Medicine2018, 59(6): 518-522. DOI： 10.13288/j.11-2166/r.2018.06.017.

摘要

目的探讨针刺人迎穴治疗高血压病的可能作用机制。方法将20只自发性高血压大鼠（SHR）随机分为人迎组和模型组

每组10只。10只WKY大鼠作为空白组。人迎组每天针刺大鼠"人迎"穴20 min

每6天休息1天

共28天;模型组和空白组不做任何干预。于针刺干预第1、5、9、13、17、21、25和28天测量血压

实验结束后取材下丘脑进行基因芯片分析

并采用PCR技术检测关键基因5-羟色胺1A受体（5-HT1A）与胱硫醚β合成酶（CBS）mRNA表达。结果与空白组比较

模型组大鼠收缩压在第1、5、9、13、17、21、25、28天升高（P<0.01）;与模型组比较

人迎组在第5

28天收缩压降低（P<0.05或P<0.01）。与本组前一观察点比较

模型组第21、25、28天血压均升高

人迎组仅第21天血压升高（P<0.05）。与空白组比较

模型组129个基因上调

243个基因下调（P<0.05）;与模型组比较

人迎组167个基因上调

188个基因下调（P<0.05）。与空白组比较

模型组5-HT1A mRNA、CBS mRNA表达下降（P<0.01）;与模型组比较

人迎组5-HT1A mRNA、CBS mRNA表达升高（P<0.01）。结论针刺"人迎"穴对于降低SHR血压有效

影响下丘脑的基因表达如促进神经递质和交感神经的兴奋性等可能是其作用机制。

Abstract

Objective To explore the possible mechanism of needling Point Renying（ ST 9） in treating hypertension. Methods Twenty spontaneous hypertension rats（ SHR） were randomized into a Renying group and a model group（ n = 10 in each group）. Another ten Wistar-Kyoto（ WKY） rats were set as a blank group. The rats in Renying group were needled at Point "Renying（ ST9） "for 20 min each day

taking one day off every 6 days

totally for 28 days. The model group and the blank group were given no intervention. Rats’ blood pressure was measured on the 1 st

5 th

9 th

13 th

17 th

21 st

25 th and 28 th day of needling. At the end of the experiment

hypothalami was collected to make gene chip analysis. The expressions of key gene 5-hydroxytryptamine 1 A receptor（ 5-HT1 A） and Cystathionine β synthetase（ CBS） mRNA were detected with polymerase chain reaction（ PCR） technology. Results Compared with that in the blank group

systolic pressure of rats in the model group increased on the 1 st

5 th

9 th

13 th

17 th

21 st

25 th and 28 th day（ P < 0. 01）. Compared with that in the model group

systolic pressure of rats in the Renying group decreased on the 5 th

9 th

13 th

21 st

25 th and 28 th day（ P < 0. 05 or P < 0. 01）. Compared with that at the previous time point in the same group

blood pressure in the model group increased on the 21 st

25 th and 28 th day

while blood pressure in the Renying group increased only on the 21 st day（ P < 0. 05）. Compared with those in the blank group

129 genes were up-regulated

and 243 genes were down-regulated in the model group（ P <0. 05）. Compared with those in the model group

167 genes were up-regulated

and 188 genes were down-regulated in the Renying group（ P < 0. 05）. Compared with those in the blank group

the expressions of 5-HT1 A mRNA and CBS mRNA in the model group decreased（ P < 0. 01）. Compared with those in the model group

the expressions of5-HT1 A mRNA and CBS mRNA in the Renying group increased（ P < 0. 01）. Conclusion Needling Point"Renying（ ST9） "seems to be effective in decreasing blood pressure of SHR. Influencing hypothalamus gene expression

such as promoting excitability of neurotransmitters and sympathetic nerves

might be the potential mechanism.

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