Objective To explore the potential mechanism of Longzhi Tang（ 龙蛭汤） promoting proliferation of human umbilical vein endothelial cells（ HUVECs）. Methods The HUVECs were made autophagy models by 400μmol/L perhydrol（ H

2

O

2

） intervention for 4 hours. The impact of serum containing Longzhi Tang on proliferation in the model group was examined by Cell Counting Kit-8（ CCK-8） method. The expressions of HUVECs Autophagy microtubule-associated protein light chain 3Ⅱ（ LC3-Ⅱ） and Bcl-2 homologous domain protein（ Beclin-1） were examined. Results Compared with the normal group

the difference of proliferation absorbance（ OD） value and relative multiplication rate in the blank group was not significant（ P

>

0. 05）. Compared with the blank group and the normal group

HUVECs proliferation OD value and relative multiplication rate in the model group decreased; the expressions of LC3-Ⅱ and Beclin-1 increased（ P

<

0. 05）. Compared with the model group

HUVECs proliferation OD value

relative multiplication rate and the expressions of LC3-Ⅱ and Beclin-1 in the Longzhi Tang group increased（ P

<

0. 05）. Cells in the normal group and the blank serum group had no obvious changes. The bilayer membrane structures and the number of vacuoles in the cytoplasm in the model group reduced. In the Longzhi Tang group

after24 h

the bilayer membrane structures in the cytoplasm increased

bending extension

packing partial organelle.Conclusion Serum containing Longzhi Tang might have obvious protective effect on HUVECs damage cells induced by H

2

O

2

which might maintain the integrity of cell morphology and promote cell proliferation. The mechanism might relate to up-regulating cell autophagy level.