Effects of Zuogui Pills（左归丸） on Iron Overload in Osteoporosis Model Rats

LIU Meijie; WU Jiaying; LI Yan; WANG Shaojun; ZHAO Hongyan; LIU Hong; PAN Jinghua; ZHANG Zhiguo; WANG Wenlai; JU Dahong

doi:10.13288/j.11-2166/r.2018.09.015

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Effects of Zuogui Pills（左归丸） on Iron Overload in Osteoporosis Model Rats

更新时间：2021-11-10

- Effects of Zuogui Pills（左归丸） on Iron Overload in Osteoporosis Model Rats
- Journal of Traditional Chinese Medicine Vol. 59, Issue 9, Pages: 777-780(2018)
- 作者机构：
  
  1. 中国中医科学院医学实验中心
  2. 中国中医科学院中医基础理论研究所
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2018.09.015
  CLC： R259
- Published：2018
- 稿件说明：
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[1]刘梅洁,吴佳莹,李艳,王少君,赵宏艳,刘红,潘静华,张治国,汪文来,鞠大宏.左归丸对骨质疏松症模型大鼠铁过载的影响[J].中医杂志,2018,59(09):777-780.

LIU Meijie, WU Jiaying, LI Yan, et al. Effects of Zuogui Pills（左归丸） on Iron Overload in Osteoporosis Model Rats[J]. Journal of Traditional Chinese Medicine2018, 59(9): 777-780.
[1]刘梅洁,吴佳莹,李艳,王少君,赵宏艳,刘红,潘静华,张治国,汪文来,鞠大宏.左归丸对骨质疏松症模型大鼠铁过载的影响[J].中医杂志,2018,59(09):777-780. DOI： 10.13288/j.11-2166/r.2018.09.015.

LIU Meijie, WU Jiaying, LI Yan, et al. Effects of Zuogui Pills（左归丸） on Iron Overload in Osteoporosis Model Rats[J]. Journal of Traditional Chinese Medicine2018, 59(9): 777-780. DOI： 10.13288/j.11-2166/r.2018.09.015.

摘要

目的探讨左归丸治疗骨质疏松症的可能作用机理。方法将60只雌性SD大鼠随机分为空白对照组、假手术组、模型组、西药组、左归丸组各12只。模型组、西药组和左归丸组大鼠行双侧卵巢切除术以制作骨质疏松症模型。造模后左归丸组给予左归丸0.968 g/100 g灌胃

西药组给予戊酸雌二醇片0.018 mg/100 g灌胃

空白对照组、假手术组和模型组给予纯净水1 ml/100 g灌胃。每日1次

每周连续给药6次

休息1天

共给药3个月。给药结束后检测各组大鼠腰椎骨密度

股骨最大载荷、弯曲强度、弹性模量

肝组织中铁离子水平及铁调素蛋白表达

胫骨骨髓中骨保护素（OPG）及细胞核因子κB受体活化因子配体（RANKL）蛋白表达。结果与假手术组比较

模型组大鼠腰椎骨密度及股骨最大载荷、弯曲强度、弹性模量水平均降低;肝组织中铁离子水平升高

铁调素蛋白降低;胫骨骨髓中OPG蛋白表达降低

RANKL蛋白表达增高（P<0.05或P<0.01）。与模型组比较

左归丸组和西药组大鼠腰椎骨密度及股骨的最大载荷、弯曲强度、弹性模量升高

肝组织铁离子水平降低

铁调素蛋白表达升高;胫骨骨髓中OPG蛋白表达升高

RANKL蛋白表达下降（P<0.05或P<0.01）。结论左归丸可提高肝脏分泌铁调素水平

进而对OPG/RANKL信号通路进行调节

最终降低破骨细胞活性

减少骨量丢失

保护骨组织

这可能是其治疗骨质疏松症的作用机理之一。

Abstract

Objective To explore the possible mechanism of Zuogui Pills（左归丸） in the treatment of osteoporosis. Methods Sixty female Sprague-Dawley rats were randomly divided into control group

sham operation group

model group

western medicine group and Zuogui Pills group

with 12 rats in each. Rats in the model group

western medicine group

and Zuogui Pills group were subjected to bilateral oophorectomy to establish osteoporosis model. After modeling

Zuogui Pills group was given Zuogui Pills 0. 968 g/100 g gavage

and western medicine group was given 0. 018 mg/100 g gavage estradiol valerate tablets. Blank control group

sham operation group and model group were given purified water 1 ml/100 g gavage. Once a day

6 days in every week for 3 months. The lumbar vertebral bone density

femur maximum load

flexural strength

elastic modulus

iron ion levels and hepcidin protein expression in rat liver

osteoprotegerin（OPG） and receptor activator of nuclear factor kappa-Β ligand（RANKL） in tibia bone marrow were measured after medication finished. Results Compared with the sham operation group

the bone mineral density and femur maximum load

flexural strength

and elastic modulus of the lumbar vertebrae in the model group were decreased. The level of iron in the hepatic tissue was increased and Hepcidin protein was decreased. The expression of OPG protein in the tibia bone marrow was decreased

and RANKL protein expression increased（P < 0. 05 or P < 0. 01）. Compared with the model group

the lumbar bone mineral density and femur maximum load

flexural strength

and elastic modulus of the Zuogui Pills group and the western medicine group increased.The iron ion level in the liver tissue decreased

and Hepcidin protein expression increased. OPG protein expression in the tibia bone marrow was increased

and RANKL protein expression decreased（P < 0. 05 or P < 0. 01）. Conclusion Zuogui Pills can increase the level of Hepcidin secreted by the liver

then regulate the OPG/RANKL signaling pathway

ultimately reduce osteoclast activity

reduce bone loss and protect bone tissue. This may be one of the mechanism in treating osteoporosis.

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Related Institution

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Zhejiang University of Chinese Medicine

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