Objective To explore the possible mechanism of Kangxianling Fang（抗纤灵方） in the treatment of renal fibrosis. Methods Mice with blood pressure < 120 mm Hg were randomly divided into normal group

surgical group

model group

Kangxianling Fang group

and fosinopril group

with 12 mice in each group. Except for the normal group and surgical group

mice in the other groups were treated with unilateral nephrectomy and micro-osmotic pump perfusion of angiotensin Ⅱ（Ang Ⅱ） to establish model of renal fibrosis. The surgical group was only given unilateral nephrectomy

and micro-osmotic pump perfusion of 0. 9% sodium chloride solution. After implantation of the micro-osmotic pump

Kangxianling Fang group was given intragastric administration of 2. 3 g/100 g Kangxianling Fang. The fosinopril group was given 0. 03 g/100 g fosinopril tablets by gavage. The normal group

surgical group and model group were given tap water gavage. Each group was given once a day

0. 5 ml each time for 4 weeks. Afterwards

the expressions of CD68

CD45

and vascular cell adhesion molecule-1（VCAM-1） in the kidney of mice were detected by immunohistochemistry and Western Blot. Results Immunohistochemistry results showed that compared with the model group

the expression gray value of CD68

CD45

and VCAM-1 in the renal tissues of the mice in Kangxianling Fang group and fosinopril group were all decreased to varying degrees（P < 0. 05 or P < 0. 01）. The expression gray value of VCAM-1 in renal tissue of mice in Kangxianling Fang group was higher than fosinopril group（P < 0. 05）. Western Blot assay results showed that compared with the model group

the expression of CD68 in the renal tissue of mice in Kangxianling Fang group and fosinopril group was decreased（P < 0. 01）

and there was no significant difference in the expression of CD45 compared with model group（P > 0. 05）. The expression of VCAM-1 in the renal tissue of mice in fosinopril group was lower than that in the model group and Kangxianling Fang group（P <0. 05 or P < 0. 01）

but the difference was not statistically significant in the expression of VCAM-1 of Kangxianling Fang group when compared with model group（P > 0. 05）. Conclusion Kangxianling Fang could reduce the expression of CD68

CD45 and VCAM-1 in the renal tissue of renal fibrosis mice

which may be one of the mechanisms in treating renal fibrosis.