In the paper

the formation and evolution of some viscera such as yolk sac

kidney

heart

lung

brain and some organs such as mouth

nose

eye and tongue during embryogenesis were traced

and it was contrasted to and analyzed with the related description of the concept and function of the spleen

kidney

heart and brain

and the opening of orifices of five zang viscera in Inner Canon of Yellow Emporor（ 《黄帝内经》）

we found that the same zang fu system comes from the same source in the embryo

and the five zang viscera and opening of orifices were due to the same time or the closely location in the embryogenesis

which was the embryology principle of the formation of the zang fu system. The theory of visceral manifestation has embryology basis. A research method called developmental tracing of zang fu and meridians was put forward by combining the visceral manifestation and embryology

which was to trace the origin of zang fu and meridians

and the connections between zang fu during embryonic development.On this basis

the physiological function and pathological changes of the zang fu after birth was studied

in order to discover the scientific basis and connotation of visceral manifestation and essence of meridian.