Effect of Tianma Gouteng Yin （天麻钩藤饮） on Content of α-synuclein in Striatum and Expression of Autophagy Protein Beclin1 and LC3B in Pakinson’s Disease Model Rats

PENG Wei; ZHANG Lijuan; ZHANG Qian; WANG Kangfeng; LI Siyi; YANG Jie; WANG Haiyan

doi:10.13288/j.11-2166/r.2018.14.014

您当前的位置：

首页 >

文章列表页 >

Effect of Tianma Gouteng Yin （天麻钩藤饮） on Content of α-synuclein in Striatum and Expression of Autophagy Protein Beclin1 and LC3B in Pakinson’s Disease Model Rats

更新时间：2021-11-10

- Effect of Tianma Gouteng Yin （天麻钩藤饮） on Content of α-synuclein in Striatum and Expression of Autophagy Protein Beclin1 and LC3B in Pakinson’s Disease Model Rats
- Journal of Traditional Chinese Medicine Vol. 59, Issue 14, Pages: 1228-1231(2018)
- 作者机构：
  
  1. 山东中医药大学附属医院
  2. 陕西中医药大学针灸推拿学院
  3. 山东中医药大学
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2018.14.014
  CLC： R-332;R285.5
- Published：2018
- 稿件说明：
移动端阅览
[1]彭伟,张立娟,张倩,王康锋,李思毅,杨洁,王海燕.天麻钩藤饮对帕金森病模型大鼠纹状体α-突触核蛋白含量及自噬相关蛋白Beclin1、LC3B表达的影响[J].中医杂志,2018,59(14):1228-1231.

PENG Wei, ZHANG Lijuan, ZHANG Qian, et al. Effect of Tianma Gouteng Yin （天麻钩藤饮） on Content of α-synuclein in Striatum and Expression of Autophagy Protein Beclin1 and LC3B in Pakinson’s Disease Model Rats[J]. Journal of Traditional Chinese Medicine2018, 59(14): 1228-1231.
[1]彭伟,张立娟,张倩,王康锋,李思毅,杨洁,王海燕.天麻钩藤饮对帕金森病模型大鼠纹状体α-突触核蛋白含量及自噬相关蛋白Beclin1、LC3B表达的影响[J].中医杂志,2018,59(14):1228-1231. DOI： 10.13288/j.11-2166/r.2018.14.014.

PENG Wei, ZHANG Lijuan, ZHANG Qian, et al. Effect of Tianma Gouteng Yin （天麻钩藤饮） on Content of α-synuclein in Striatum and Expression of Autophagy Protein Beclin1 and LC3B in Pakinson’s Disease Model Rats[J]. Journal of Traditional Chinese Medicine2018, 59(14): 1228-1231. DOI： 10.13288/j.11-2166/r.2018.14.014.

摘要

目的探讨天麻钩藤饮治疗帕金森病的可能作用机制。方法采用6-羟基多巴胺注射法建立帕金森病大鼠模型

造模成功后将大鼠随机分为模型组、西药组和中药高、低剂量组

另设假手术组

每组10只。中药低、高剂量组分别给予天麻钩藤饮11.88、47.52 g/（kg·d）灌胃

西药组予多巴丝肼片75 mg/（kg·d）灌胃

模型组、假手术组予等量生理盐水灌胃

共干预8周。治疗后采用HE染色法观察大鼠脑组织纹状体形态学变化

免疫组织化学法测定纹状体中α-突触核蛋白含量及自噬相关蛋白Beclin1、LC3B表达情况。结果假手术组中纹状体神经元细胞密集

形态正常;模型组神经元的损伤较为严重

神经细胞数量明显减少;中药高、低剂量组和西药组纹状体细胞形态与模型组比较均有不同程度的改善。与假手术组比较

模型组大鼠纹状体内α-突触核蛋白灰度值下降

Beclin1、LC3B灰度值升高（P<0.05）。与模型组比较

中药高剂量组和西药组α-突触核蛋白灰度值升高

中药高、低剂量组和西药组Beclin1和LC3B灰度值降低（P<0.05）。与西药组比较

中药高剂量组α-突触核蛋白灰度值升高

Beclin1灰度值降低（P<0.05）。结论天麻钩藤饮可能通过上调纹状体神经细胞自噬活性

清除过量α-突触核蛋白

从而发挥神经保护作用。

Abstract

Objective To explore the possible mechanism of Tianma Gouteng Yin（天麻钩藤饮） in treating Parkinson’s disease. Methods A rat model of Parkinson’ s disease was established by 6-hydroxydopamine injection.After successful model establishment

the rats were randomly divided into model group

western medicine group

and high or low dose groups of traditional Chinese medicine

and a sham operation group

10 rats in each group. The low and high dose groups of traditional Chinese medicine were given Tianma Gouteng Yin 11. 88 and 47. 52 g/（ kg·d）gavage

respectively

and the western medicine group were given tobabasic tablets 75 mg/（ kg·d） gavage. The same amount of saline was given to the the model group and the sham speration group for a total of 8 weeks. HE staining was used to observe the morphological changes of brain tissue in rats. Immunohistochemistry was used to determine the content of α-synuclein in the striatum and the expression of autophagy-related proteins Beclin1 and LC3 B. Results In the sham operation group

the striatum neurons were densely packed and the morphology was normal. In the model group

the damage of the neurons was severe and the number of nerve cells was significantly reduced. The striatum cell morphology of the high

low dose of the Chinese medicine group and western medicine group was improved compared with that of the model group. Compared with the sham group

the gray value of α-synuclein in the striatum of the model group decreased

and the gray value of Beclin1 and LC3 B increased（ P < 0. 05）. Compared with the model group

the gray value of α-synuclein in the high and low dose group as well as the western medicine group increased

and the gray levels of Beclin1 and LC3 B in the high-dose

low-dose group and western medicine group decreased（ P < 0. 05）. Compared with western medicine group

the gray value of α-synuclein in high-dose group was increased

and the gray value of Beclin1 was decreased（ P < 0. 05）. Conclusion Tianma Gouteng Yin may play a neuroprotective role by scavenging excess α-synuclein and upregulating autophagy activity in striatum.

关键词

Keywords

references

查看原文

Views

633

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effects of Nourishing-Kidney Formula on Neurotrophic Factors in Different Brain Regions of Parkinson’s Disease Model Rats

Effect of Bushen Huoxue Granule （补肾活血颗粒） on Dopamine Homeostasis and ERK/CREB/VMAT2 Signaling Pathways in the Striatum in Parkinson's Disease Model Mice

Du （督） Three Needles Combined with Hand & Foot Luan （挛） Three Needles in the Treatment of Myotonia During Early-Mid Stage Parkinson's Disease： A Randomized Controlled Trial

Effects of Huayu Jiedu Formula （化瘀解毒方） on Brain Cell Autophagy and Blood Brain Barrier Permeability in Ischemic Stroke Model Rats with Binding of Stasis and Toxin Syndrome

Clinical Observation on Kidney-Enriching to Nourish the Liver Method for 18 Cases of Parkinson’s Disease

Related Author

LIN Yao

YANG Shasha

LIU Ting

ZHONG Jianan

XU Qian

HUANG Lufen

YUAN Mingzhou

LIN Youning

Related Institution

College of Integrative Medicine,Fujian University of Traditional Chinese Medicine

Academy of Integrative Medicine,Fujian University of Traditional Chinese Me dicine

Third People’s Hospital Affiliated to Fujian University of Traditional Chinese Medicine

Chinese People's Liberation Army Medical School

Department of Traditional Chinese medicine，The sixth medical center，The People's Liberation Army General Hospital

Address：No.16, Nanxiao street, Dongzhimen, Dongcheng District, Beijing Postal code：100700
Tel：010-64035632 Email：jtcmcn@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19 京公网安备11010102007558号
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰