Objective To explore the possible mechanism of Bushen Conger Fang（ 补肾聪耳方） in the treatment of drug deafness. Methods Totally 24 SD rats were randomly divided into blank group

model group

and herbal medicine group with 8 in each group. The rat model of drug deafness was induced by intramuscular injection of gentamicin sulfate 100 mg/（ kg·d） for 14 days in both the model group and the herbal medicine group. The herbal medicine group was given intragastric administration of Bushen Conger Fang 2. 2 g/（ kg·d） at the same time of model building. The blank group and model group were given normal saline 4 ml/（ kg·d） by gavage. After 14 days

the auditory brainstem response（ ABR） thresholds were observed and the mitogen-activated protein kinase（ MAPK） signaling pathway related proteins c-Jun N-terminal kinase（ JNK） and extracellular regulatory protein kinase 1（ ERK1）

expression of extracellular regulated protein kinase 2（ ERK2） were determined. Results Compared with the blank group

the ABR threshold was increased in the model group

and the expression of JNK protein in the cochlea tissue was increased

while the expression of ERK1 and ERK2 protein was decreased（ P < 0. 05）. Compared with the model group

the threshold value of ABR in the herbal medicine group was decreased

and the cochlea was decreased. The expression of JNK protein was down-regulated

while the expression of ERK1 and ERK2 protein was increased（ P < 0. 05）. Conclusion Bushen Conger Fang may improve drug deafness by regulating the expression of MAPK signaling pathway related proteins and reducing the ABR threshold.