Objective To study the biological features of animal model of myocardial infarction with qi deficiency and blood stasis syndrome and qi stagnation and blood stasis syndrome. Methods A total of 36 male Wistar rats were randomly divided into 4 groups: normal group（ n = 6）

myocardial infarction model group（ n = 10）

qi deficiency and blood stasis syndrome（ n = 10） and qi stagnation and blood stasis syndrome（ n = 10）. The models of myocardial infarction was made by ligating the anterior decending branches of the left coronary artery. On this basis

the models of myocardial infarction with qi deficiency and blood stasis syndrome were established by hunger

fatigue

cold and dampness

and the models of myocardial infarction with qi stagnation and blood stasis syndrome were established by circadian disorders

scintillation and clipper stimulation combined with ice water. The genera condition

pathologic staining of myocardium tissues of rats were observed. The platelet aggregation rate

and the levels of GMP-140

CGRP and NE were detected. Results The rats in qi deficiency and blood stasis syndrome group were burnout and lethargy

few of activities. Qi stagnation and blood stasis syndrome group was more excited and irritated

and had more activities. GMP-140

NE of 3 model groups were all higher than that of normal group. CGRP of 3 model groups were all lower than that of normal group

with statistical significance（ P < 0. 01）. GMP-140 of qi deficiency and blood stasis group and qi stagnation and blood group were higher than myocardial infarction model group. CGRP of qi deficiency and blood stasis group was lower than qi stagnation and blood group and myocardial infarction model group. NE of qi stagnation and blood group was higher than that of others（ P < 0. 01）. Infarction region of 3 model groups was pale after NBT staining. Conclusion qi deficiency and blood stasis syndrome and qi stagnation and blood stasis syndrome models established by multifactor combined methods

are combined with myocardial infarction to establish integration of disease and syndrome animal model. Biological markers are difference of between myocardial infarction of qi deficiency and blood stasis syndrome and qi stagnation and blood.