Effects of Jinkui Shenqi Pill （金匮肾气丸） on Protein Expression in Femoral Cancellous Bone Tissues of Rats with Kidney-yang Deficiency Syndrome

HUANG Lulu; ZHU Yaju; LAI Xingquan; LI Baolin; Wang Jianhui; CAI Tangyan; ZHANG Yiqiang; TENG Fangzhou; ZHAN Zhengxuan; ZHANG Ziyi; SU Youxin

doi:10.13288/j.11-2166/r.2018.21.015

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Effects of Jinkui Shenqi Pill （金匮肾气丸） on Protein Expression in Femoral Cancellous Bone Tissues of Rats with Kidney-yang Deficiency Syndrome

更新时间：2021-11-10

- Effects of Jinkui Shenqi Pill （金匮肾气丸） on Protein Expression in Femoral Cancellous Bone Tissues of Rats with Kidney-yang Deficiency Syndrome
- Journal of Traditional Chinese Medicine Vol. 59, Issue 21, Pages: 1862-1867(2018)
- 作者机构：
  
  1. 福建中医药大学康复医学院
  2. 福建中医药大学骨伤学院
  3. 福建卫生职业技术学院
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2018.21.015
  CLC： R285.5
- Published：2018
- 稿件说明：
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[1]黄露露,朱亚菊,赖兴泉,李保林,王建辉,蔡唐彦,张艺强,滕方舟,詹正烜,张子怡,苏友新.金匮肾气丸对肾阳虚证模型大鼠股骨髁松质骨差异蛋白质表达的影响[J].中医杂志,2018,59(21):1862-1867.

HUANG Lulu, ZHU Yaju, LAI Xingquan, et al. Effects of Jinkui Shenqi Pill （金匮肾气丸） on Protein Expression in Femoral Cancellous Bone Tissues of Rats with Kidney-yang Deficiency Syndrome[J]. Journal of Traditional Chinese Medicine2018, 59(21): 1862-1867.
[1]黄露露,朱亚菊,赖兴泉,李保林,王建辉,蔡唐彦,张艺强,滕方舟,詹正烜,张子怡,苏友新.金匮肾气丸对肾阳虚证模型大鼠股骨髁松质骨差异蛋白质表达的影响[J].中医杂志,2018,59(21):1862-1867. DOI： 10.13288/j.11-2166/r.2018.21.015.

HUANG Lulu, ZHU Yaju, LAI Xingquan, et al. Effects of Jinkui Shenqi Pill （金匮肾气丸） on Protein Expression in Femoral Cancellous Bone Tissues of Rats with Kidney-yang Deficiency Syndrome[J]. Journal of Traditional Chinese Medicine2018, 59(21): 1862-1867. DOI： 10.13288/j.11-2166/r.2018.21.015.

摘要

目的探讨金匮肾气丸治疗肾阳虚证的可能作用机制。方法 60只Wistar大鼠随机分为正常组20只和造模组40只

将造模成功的38只大鼠再随机分为中药组和模型组各19只

中药组大鼠给予金匮肾气丸8. 44 g/（kg·d）灌胃

正常组和模型组大鼠给予生理盐水3 ml灌胃

每日1次

连续3周。分别取正常组、模型组和中药组大鼠的股骨髁松质骨

进行总蛋白的提取与制备;采用蛋白质双向凝胶电泳技术展示各组大鼠股骨踝松质骨蛋白质组图谱

应用PDQuest 8. 0软件分析各组间差异表达蛋白点

采用基质辅助激光解析电离飞行时间质谱（MALDI-TOF-MS）初步鉴定差异表达蛋白点。结果各组大鼠股骨髁松质骨双向凝胶电泳图谱的蛋白点数目均为552个;与正常组比较

模型组有23个差异表达的蛋白质;与模型组比较

中药组有16个差异表达的蛋白质。给予金匮肾气丸溶液后

肾阳虚模型大鼠股骨髁松质骨中原来表达上调的磷酸甘油酸激酶1及表达下调的14-3-3ζ蛋白、网钙蛋白3、原肌球蛋白异构体2α-3链、原肌球蛋白α-4链、原肌球蛋白异构体α-1链、40s核糖体蛋白sa、半乳糖凝集素1、线粒体ATP合成酶亚基α、膜联蛋白A1、GDP解离抑制因子1、Ⅰ型胶原α-1链、LOC683667蛋白、膜联蛋白A5、过氧化氧化还原蛋白2和蛋白质二硫键异构酶A3均出现表达趋势的反向调整。结论金匮肾气丸对肾阳虚证的调节可能涉及股骨髁松质骨结构构成、物质转运、能量代谢和细胞增殖与凋亡等方面。

Abstract

Objective To study the mechanism of Jinkui Shenqi Pills（金匮肾气丸） on kidney-yang deficiency.Methods A total of 60 male Wistar rats were randomly divided into 20 rats in a normal group and 40 in experimental group. Thirty-eight successfully modeling rats were randomly divided into traditional Chinese medicine group and model group

with 19 rats in each group. Jinkui Shenqi Pills 8. 44 g/（ kg·d） was instragastrically given to the traditional Chinese medicine group

and 3 ml normal saline was given to the normal group and the model group

once a day for 3 weeks consecutively. Femoral cancellous bone tissues of each group were obtained to extract and prepare the total protein. Two-dimensional gel electrophoresis（ 2-DE） technique was used to display the cancellous bone proteome map of each group. PDQuest 8. 0 software was used to analyze the differentially expressed protein spots in each group. The matrix-assisted laser desorption ionization time of flight mass spectrometry（ MALDI-TOF-MS） was used to initially identify differentially expressed protein spots. Results There were 552 discernible proteins spots in each rat femoral cancellous bone total protein 2-DE gel map of 3 groups. Compared with the normal group

the model group had 23 differentially expressed proteins. Compared with the model group

traditional Chinese medicine group had 16 differentially expressed proteins. After treatment with Jinkui Shenqi Pill

the expression of up-regulated phosphoglycerate kinase 1 and the down-regulated 14-3-3ζ protein

reticulocalbin 3

myosin promyosin isomer 2α-3 chain

myosin α-4 chain

myosin isoform α-1 chain

40 s ribosomal protein sa

galactoside agglutinin 1

annexin A1

GDP dissociation inhibition factor 1

type I collagen α-1 chain

LOC683667 protein

annexin A5

peroxy redox protein 2 and protein disulfide isomerase A3 all present reserve trend adjustment. Conclusion The regulation of Jinkui Shenqi Pills on kidney-yang deficiency probably concern with the structure of femoral cancellous bone tissues

material transport

energy metabolism

proliferation and apoptosis of cells

etc.

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