Objective To explore the possible mechanism of Shugan Jianpi Huoxue Decoction（疏肝健脾活血方）（STHD） in treating hepatic fibrosis.Methods Ten SD rats were orally administered with 10 ml/kg Shugan Jianpi Huoxue Decoction（concentration 2.7 g/ml） once a day for 3 consecutive days to prepare drug-containing serum.The serum of traditional Chinese medicine is set at 5 concentrations of 5%

10%

15%

20% and 25%.The concentration of the experimental drug was screened by MTT method

and the liver fibrosis sinusoidal endothelial cells（SEC）were isolated by intraperitoneal injection of carbon tetrachloride（CCl4）.The normal rat hepatic stellate cells（HSC）and SEC were collected.In this experiment

the rats were divided into 7 groups including normal group

model group

DAPT group

NF group

Chinese medicine group

Chinese medicine plus DAPT group and Chinese medicine plus NF group.HSC was added to each group

and SEC of normal rats was added to the normal group

and SEC of liver fibrosis rats were added to the other groups for 24 hours.The supernatant was aspirated

and the DAPT group

the Chinese medicine plus DAPT group were added with Notch pathway blocker DAPT 0.2 μmol/L

and the NF group and the Chinese medicine plus NF group were added with Notch pathway agonist rhNF-κB 1 gsu/ml.The first4 groups were added with 10% FBS DMEM/12 medium

and the last 3 groups were added with pre-screened concentration Chinese medicine serum

10% FBS DMEM/12 medium.After 72 hours of culture

the MTC activity of each group was detected by MTT method

and the expression of CD31 and vWF proteins in SEC was detected by Western Blot method.Results The screening out 10% concentration of traditional Chinese medicine in serum had an inhibitory effect on cell proliferation.Compared with the normal group

the HSC activity of the model group was increased after 2

4

and 8 weeks

and the CD31 protein expression was increased after 4 and 8 weeks since model establishment（P<0.05 or P<0.01）.Compared with the model group

the HSC activity of the NF group was increased.The HSC activity

CD31 and vWF protein expression were decreased in DAPT group and Chinese medicine group（P <0.05 or P<0.01）.Compared with the NF group

the HSC activity

CD31 and vWF protein expression decreased in traditional Chinese medicine plus NF group（P<0.05 or P<0.01）.Compared with the DAPT group

the expression of HSC

CD31 and v WF protein in Chinese medicine plus DAPT group were decreased（P<0.05 or P<0.01）.Conclusion Shugan Jianpi Huoxue Decoction could inhibit HSC activity and the defenestration of SEC by interfering plu Notch pathway

which may be one of the mechanisms of its treatment of liver fibrosis.