Effects of Zuogui Jiangtang Tongmai Formulae（左归降糖通脉方） Drug-Containing Serum on Brain Microvascular Endothelial Cells Injury Induced by Advanced Glycation End Products

LI Yujia; LI Dingxiang; MAZHONG Danni; ZHOU Han; DENG Yihui

doi:10.13288/j.11-2166/r.2019.03.014

您当前的位置：

首页 >

文章列表页 >

Effects of Zuogui Jiangtang Tongmai Formulae（左归降糖通脉方） Drug-Containing Serum on Brain Microvascular Endothelial Cells Injury Induced by Advanced Glycation End Products

更新时间：2021-11-10

- Effects of Zuogui Jiangtang Tongmai Formulae（左归降糖通脉方） Drug-Containing Serum on Brain Microvascular Endothelial Cells Injury Induced by Advanced Glycation End Products
- Journal of Traditional Chinese Medicine Vol. 60, Issue 3, Pages: 237-241(2019)
- 作者机构：
  
  湖南中医药大学中西医结合学院
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2019.03.014
  CLC： R285.5
- Published：2019
- 稿件说明：
移动端阅览
[1]李钰佳,李定祥,马钟丹妮,周韩,邓奕辉.左归降糖通脉方含药血清对晚期糖基化终末产物诱导脑微血管内皮细胞损伤的影响[J].中医杂志,2019,60(03):237-241.

LI Yujia, LI Dingxiang, MAZHONG Danni, et al. Effects of Zuogui Jiangtang Tongmai Formulae（左归降糖通脉方） Drug-Containing Serum on Brain Microvascular Endothelial Cells Injury Induced by Advanced Glycation End Products[J]. Journal of Traditional Chinese Medicine2019, 60(3): 237-241.
[1]李钰佳,李定祥,马钟丹妮,周韩,邓奕辉.左归降糖通脉方含药血清对晚期糖基化终末产物诱导脑微血管内皮细胞损伤的影响[J].中医杂志,2019,60(03):237-241. DOI： 10.13288/j.11-2166/r.2019.03.014.

LI Yujia, LI Dingxiang, MAZHONG Danni, et al. Effects of Zuogui Jiangtang Tongmai Formulae（左归降糖通脉方） Drug-Containing Serum on Brain Microvascular Endothelial Cells Injury Induced by Advanced Glycation End Products[J]. Journal of Traditional Chinese Medicine2019, 60(3): 237-241. DOI： 10.13288/j.11-2166/r.2019.03.014.

摘要

目的探讨左归降糖通脉方治疗糖尿病血管并发症的可能作用机制。方法将30只大鼠随机分为空白组、中药组和西药组

每组10只。中药组给予左归降糖通脉方36 g/（kg·d）灌胃

西药组给予尼莫地平片18. 35 mg/（kg·d）及格列齐特缓释片27. 5 mg/（kg·d）灌胃

空白组予以4 ml蒸馏水灌胃

均每日1次

连续5天

制备含药血清。将晚期糖基化终末产物（AGEs）设为50、100、200、400 mg/L 4个浓度组

分别作用12、24、48 h 3个时间点

观察AGEs对大鼠脑微血管内皮细胞（BMECs）形态结构改变的影响来筛选最佳干预浓度和时间。将左归降糖通脉方含药血清分为2. 5%、5%、10%3个浓度组

作用于BMECs 24 h后

测定BMECs增殖率来确定左归降糖通脉方中药含药血清的最佳作用浓度。再将BMECs分为空白组、模型组、中药组、西药组

中药组和西药组分别加入相应含药血清进行干预。检测各组细胞上清液中白细胞介素1β（IL-1β）、细胞间黏附分子1 （ICAM-1）、血管细胞黏附分子1 （VCAM-1）的含量。结果AGEs的最佳干预浓度和时间点为200 mg/L作用24 h

中药含药血清的最佳作用浓度为5%。与空白组比较

模型组上清液中IL-1β、ICAM-1、VCAM-1含量明显升高（P <0. 05）;与模型组比较

中药组和西药组IL-1β、ICAM-1、VCAM-1明显下调（P <0. 05）。结论左归降糖通脉方能下调IL-1β、ICAM-1、VCAM-1表达

从而抑制炎症反应

可能是其治疗糖尿病血管并发症的作用机制之一。

Abstract

Objective To investigate the possible mechanism of Zuogui Jiangtang Tongmai Formulae（ ZGJTTMF）（左归降糖通脉方） in the treatment of diabetic vascular complications. Methods Thirty rats were randomly divided into the blank group

the Chinese medicine group and the western medicine group

with 10 rats in each group. The Chinese medicine group was intragastrically given ZGJTTMF 36 g/（ kg ·d）

and the western medicine group was given nimodipine tablets 18. 35 mg/（ kg·d） and gliclazide sustained-release tablets 27. 5 mg/（ kg·d） by gavage. The blank group was intragastrically administered with 4 ml of distilled water. Rats were all given once a day for 5 consecutive days to prepare the drug-containing serum. The advanced glycation end products（ AGEs） were set to four concentrations of 50

100

200

and 400 mg/L for 12

and 48 h. Effects of AGEs on morphological structural changes of rat brain microvascular endothelial cells（ BMECs） were observed to screen for optimal intervention concentrations and times. The drug-containing serum of ZGJTTMF was divided into three concentration groups of2. 5%

5% and 10%. After 24 hours of action on BMECs

the proliferation rate of BMECs was measured to determine the optimal action concentration of serum containing the drug of ZGJTTMF. The BMECs were divided into the blank group

the model group

the Chinese medicine group and the western medicine group. The Chinese medicine group and the western medicine group were respectively added with corresponding drug-containing serum for intervention. The contents of interleukin-1β（ IL-1β）

intercellular adhesion molecule 1（ ICAM-1） and vascular cell adhesion molecule 1（ VCAM-1） in the supernatant of each group were detected. Results The optimal concentration and time point of AGEs was 200 mg/L for 24 h

and the optimal concentration of Chinese medicine containing serum was 5%. Compared with the blank group

the contents of IL-1β

ICAM-1 and VCAM-1 in the supernatant of the model group were significantly increased（ P < 0. 05）. Compared with the model group

IL-1β

ICAM-1 and VCAM-1 in the Chinese medicine group and the western medicine group were significantly down-regulated（ P < 0. 05）.Conclusion ZGJTTMF can down-regulate the expression of IL-1β

ICAM-1 and VCAM-1

thus inhibiting the inflammatory response

which may be one of the mechanisms of its treatment of diabetic vascular complications.

关键词

Keywords

references

查看原文

Views

233

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of Electroacupuncture on Metabolism-Inflammation Network in Patients with Simple Obesity：A Randomized Controlled Trial

Effect of Qingfei Shenshi Decoction （清肺渗湿汤） Combined with Western Medicine on Clinical Effectiveness and Immune Function for Patients with Bronchial Asthma of Heat Wheezing Syndrome

Modified Shentong Zhuyu Decoction（身痛逐瘀汤） as an Adjuvant Therapy for Rheumatoid Arthritis with Interstitial Lung Disease and Blood Stasis Obstruction Syndrome：A Prospective Cohort Study

Effects of Zhimu （Anemarrhena asphodeloides）-Huangbai （Phellodendron amurense） Medicinal Pair on Femoral Microstructure and Osteogenic-Adipogenic Differentiation in Ovariectomized Osteoporosis Model Rats

Shugan Jianpi Granules （疏肝健脾颗粒） Combined with Chushi Zhiyang Oinement （除湿止痒软膏） for Atopic Dermatitis with Sleep Disturbance of Liver Constraint and Spleen Deficiency Syndrome： A Randomized Controlled Trial

Related Author

SHU Qi

PENG Qiumei

XU Zhihao

FANG Shanshan

SHAO Wenjing

CHENG Hongliang

HUANG Rilong

YU Qing

Related Institution

Institute of Xin'an Medicine and Modernization of Traditional Chinese Medicine，Institute of Health，Hefei Comprehensive National Science Center

Anhui Provincial Key Laboratory of Meridian and Zang-Fu Organs Interrelationships

The First Affiliated Hospital of Anhui University of Chinese Medicine

The Second Affiliated Hospital of Anhui University of Chinese Medicine

Anhui University of Chinese Medicine

Address：No.16, Nanxiao street, Dongzhimen, Dongcheng District, Beijing Postal code：100700
Tel：010-64035632 Email：jtcmcn@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19 京公网安备11010102007558号
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰