Objective To investigate the possible action mechanism of Danggui Shaoyao Powder（当归芍药散）in the treatment of Alzheimer’s disease（AD）. Methods The network pharmacology research method was used to predict the target of Danggui Shaoyao Powder and AD using traditional Chinese medicine system pharmacology（TCMSP） database and analysis plaform. The STRING online database was used to construct a protein interaction network for the target proteins at the intersection of the two sides

and visualized in Cytoscape 3. 6. 1 software. The gene name of the target protein was searched in the Unitprot database

and the genes were found to perform gene ontology（GO）enrichment analysis and KEGG pathway enrichment analysis using the DAVID tool to explore the possible biological functions and pathways of potential targets. Results Totally 38 compounds were screened from Danggui Shaoyao Powder and involved 9 targets for the treatment of AD. The results of network analysis indicated that the target may play a role through cholinergic synaptic transmission

acetylcholine receptor signaling pathway

nervous system developmental

signal transduction and other biological processes

combined with G protein-coupled acetylcholine receptor activity

peroxidase activity

acetylcholine ligand-gated ion signaling pathway

amyloid β-binding protein and other molecular functions; The mechanism is mainly related to the cholinergic synaptic pathway

the neuroligand receptor interaction pathway

the calcium signaling pathway

the signaling pathway related to nicotine addiction

and the PI3 K-Akt signaling pathway. Conclusion Danggui Shaoyao Powder has the characteristics of multiple targets and multiple pathways in the treatment of AD.