Objective To investigate the possible mechanism of Shenfu Yixin Granules（ 参附益心颗粒） in treating chronic cardiac failure. Methods H

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cells were cultured for 24 h with Shenfu Yixin Granules at different concentrations（ 0. 1

0. 2

0. 3

0. 4

0. 5

0. 6

0. 9

1. 2 mg/ml）

and the cell activity was determined by MTT colorimetric assay to screen for drug concentrations in subsequent experiments. The H

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cells were divided into normal group（ normal culture）

model group（ hypoxia 6 h）

trimetazidine group( 1 × 10

-4

mol/L trimetazidine + hypoxia 6 h)

coenzyme Q10 group( 1 × 10

-4

mol/L coenzyme Q10 + hypoxia 6 h)

low dose Chinese medicine group（ 0. 25 mg/ml Shenfu Yixin Granules + hypoxia 6 h）

and high dose Chinese medicine group（ 0. 5 mg/ml Shenfu Yixin Granules + hypoxia 6 h）. The protein expression levels of adenosine triphosphate（ ATP）

fatty acid transporter（ FAT）

carnitine lipid acetyltransferase 1（ MCPT-1）

medium chain Lipoxycoenzyme A dehydrogenase（ MCAD）

phosphate activated protein Kinase（ AMPK）

pyruvate dehydrogenase（ PDH）

glucose transporter 4（ GLUT-4）

adenylate transporter（ ANT）

creatine kinase（ CK） in the cardiomyocytes of each group were detected. The ultrastructure changes of H

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cardiomyocytes in each group were observed. H

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cells were divided into normal group

model group and Chinese medicine group（ 0. 5 mg/ml Shenfu Yixin Granules + hypoxia 6 h）. Mitochondrial membrane potential was detected by JC-1 staining. Results There was no cytotoxicity in the 0. 5 mg/ml Shenfu Yixin Granules group and the lower concentration group

which was suitable for administration of 0. 5 mg/ml Shenfu Yixin Granules in Chinese medicine group. Compared with the normal group

the ATP content and FAT

MCPT-1

MCAD

GLUT-4

ANT

CK protein contents in H

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cells in the model group were significantly decreased

and AMPK

PDH protein contents were significantly increased（ P

<

0. 05）. Compared with the model group

the ATP content and FAT

MCPT-1

MCAD

GLUT-4

ANT

CK protein contents in the cardiomyocytes of Chinese medicine group and coenzyme Q10 group were significantly increased（ P

<

0. 05）. In the normal group

the mitochondria structure was normal. The membrane of the model group was broken

the mitochondria were swollen

and the boundary was blurred. Compared with the model group

the cell damage in the Chinese medicine group was obviously alleviated

the cell membrane was intact

and the mitochondrial structure was clear. JC-1 staining showed that the Chinese medicine group could improve the decrease of mitochondrial membrane potential. Conclusion Shenfu Yixin Granules may increase the expression of ATP

GLUT-4

ANT

FAT

MCPT-1

MCAD

CK protein

decreased the expression of AMPK and PDH protein

improve mitochondrial membrane potential of H

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cardiomyocytes induced by hypoxia

so as to play the role for treating chronic cardiac failure.