Objective To observe the effects of Yougui Decoction（ 右归饮） on cartilage differentiation of rabbit bone marrow mesenchymal stem cells（ BMSCs） and the effects of its induced BMSCs/fibrin glue complex on the repair of rabbit knee articular cartilage defects. Methods Rabbit tibia and femur bone marrow cells were isolated and subcultured to obtain rabbit BMSCs. Rabbit BMSCs were divided into a control group

an induction group and Yougui Decoction low

medium and high dose groups. The expression of type Ⅱ collagen and aggrecan mRNA in BMSCs cells and the expression of the protein was detected. The concentration of the subsequent experiment of Yougui Decoction was screened. Rabbits with knee joint cartilage injury were randomly divided into 5 groups

with 6 rabbits in each group. Among them

the Yougui Decoction group was implanted with the right concentration of BMSCs/fibrin glue complex for 1 week into the rabbit cartilage defect site; TGF-β1 group implanted BMSCs/fibrin glue complex induced by transforming growth factor β1（ TGF-β1） for 1 week into rabbit cartilage defect site; fibrin glue group implanted fibrin glue into rabbit cartilage defect part; the model group’s defect was put aside; another normal control group was set up without any treatment. After 8 weeks of intervention in each group

the cartilage defects of the knee joint were taken

and the cartilage injury repair effect was evaluated by gross observation

HE staining and Wakitani score.Results Compared with the control group

the mRNA and protein levels of type Ⅱ collagen and aggrecan were significantly up-regulated in the middle and high dose groups of Yougui Decoction

and the effect was the best in the high-dose group of Yougui Decoction（ P < 0. 05）. Therfore

Yougui Decoction 400 mg/ml was selected for subsequent experiments. In the normal control group

the knee joints were normal in shape and smooth

and there was no damage. In the model group

the knee joint articular cartilage defect was dark red

and the cartilage defect was irregular.No chondrocytes were seen

and only a small amount of fibrous tissue and cartilage tissue were observed. The Wakitani score was increased（ P < 0. 05）. In the fibrin glue group

the knee articular cartilage defect area was smaller

the cartilage defect had repair filler

and the Wakitani score was decreased（ P < 0. 05）. Compared with the fibrin glue group

the knee joint articular cartilage defects in the Yougui Decoction group and the TGF-β1 group were basically repaired intact. The HE staining at the repair site was close to that of the surrounding normal tissues. A large number of chondrocytes were seen

and the cartilage lacuna structure appeared. The Wakitani score pof cartilage tissue was reduced（ P < 0. 05）. Conclusion Yougui Decoction can induce chondrogenic differentiation of rabbit BMSCs

and the induced BMSCs/fibrin glue complex can promote the repair of rabbit knee articular cartilage defects.