Correlation between Lung and Kidney Qi Deficiency Syndrome and Angiotensin Converting Enzyme Gene Single Nucleotide Polymorphism in Patients with Stable Chronic Obstructive Pulmonary Disease

WU Xiaozheng; WANG Zhenxing; WANG Yilan; KANG Wenlin; QING Shihua; WANG Fei; CHEN Min

doi:10.13288/j.11-2166/r.2019.15.009

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Correlation between Lung and Kidney Qi Deficiency Syndrome and Angiotensin Converting Enzyme Gene Single Nucleotide Polymorphism in Patients with Stable Chronic Obstructive Pulmonary Disease

更新时间：2021-11-10

- Correlation between Lung and Kidney Qi Deficiency Syndrome and Angiotensin Converting Enzyme Gene Single Nucleotide Polymorphism in Patients with Stable Chronic Obstructive Pulmonary Disease
- Journal of Traditional Chinese Medicine Vol. 60, Issue 15, Pages: 1299-1303(2019)
- 作者机构：
  
  1. 贵州中医药大学基础医学院
  2. 成都中医药大学临床医学院
  3. 成都市第三人民医院
  4. 贵州中医药大学基础医学院成都中医药大学临床医学院成都市第三人民医院
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2019.15.009
  CLC： R259
- Published：2019
- 稿件说明：
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[1]吴孝政,王振兴,王依澜,康雯霖,卿世花,王飞,陈敏.慢性阻塞性肺疾病稳定期患者肺肾气虚证与血管紧张素转换酶基因单核苷酸多态性的相关性[J].中医杂志,2019,60(15):1299-1303.

WU Xiaozheng, WANG Zhenxing, WANG Yilan, et al. Correlation between Lung and Kidney Qi Deficiency Syndrome and Angiotensin Converting Enzyme Gene Single Nucleotide Polymorphism in Patients with Stable Chronic Obstructive Pulmonary Disease[J]. Journal of Traditional Chinese Medicine2019, 60(15): 1299-1303.
[1]吴孝政,王振兴,王依澜,康雯霖,卿世花,王飞,陈敏.慢性阻塞性肺疾病稳定期患者肺肾气虚证与血管紧张素转换酶基因单核苷酸多态性的相关性[J].中医杂志,2019,60(15):1299-1303. DOI： 10.13288/j.11-2166/r.2019.15.009.

WU Xiaozheng, WANG Zhenxing, WANG Yilan, et al. Correlation between Lung and Kidney Qi Deficiency Syndrome and Angiotensin Converting Enzyme Gene Single Nucleotide Polymorphism in Patients with Stable Chronic Obstructive Pulmonary Disease[J]. Journal of Traditional Chinese Medicine2019, 60(15): 1299-1303. DOI： 10.13288/j.11-2166/r.2019.15.009.

摘要

目的探讨血管紧张素转换酶（ACE）基因单核苷酸多态性（SNP）插入/缺失(I/D（rs4646994）与慢性阻塞性肺疾病（COPD）稳定期肺肾气虚证的相关性。方法选取COPD稳定期患者95例为病例组

依据中医辨证标准分为肺肾气虚证组（37例）和非肺肾气虚证组（58例）

另选取同期健康体检者82例为对照组。提取所有受试者全基因组DNA并采用PCR法对ACE基因SNP （I/D）进行检测

采用Pearson相关性分析进行组间比较

最后多态性采用SNP序列查询进行验证。结果 95例病例组患者II、ID、DD基因型分布频率分别为44例（46. 32%）、38例（40. 00%）、13例（13. 68%）

对照组分别为33例（40. 24%）、40例（48. 78%）、9例（10. 98%）。肺肾气虚证组分别为16例（43. 24%）、17例（45. 95%）、4例（10. 81%）

非肺肾气虚证组分别为28例（48. 28%）、21例（36. 21%）、9例（15. 52%）。各组受试者ACE基因SNP （I/D）（rs4646994）组间相关性分析结果差异均无统计学意义（P>0. 05）。SNP序列查询结果示

rs4646994是一个289bp重复序列的I/D突变

但已知的突变频率小于1%。结论 ACE基因SNP （I/D）（rs4646994）作为COPD稳定期肺肾气虚证的遗传标记物证据尚不足

仍需要大样本进行验证。

Abstract

Objective To investigate the relevance between angiotensin-converting enzyme（ACE） gene single nucleotide polymorphism（SNP） and chronic obstructive pulmonary disease（COPD） of stable period with lung and kidney qi deficiency syndrome. Methods A total of 95 patients with stable COPD were selected as the case group.According to the traditional Chinese medicine（TCM） syndrome differentiation criteria

they were divided into a lung and kidney qi deficiency syndrome group（37 cases） and a non-lung kidney qi deficiency syndrome group（58 cases）. The other 82 healthy people were selected as the control group. The genomic DNA of all subjects was extracted and the ACE gene SNP（I/D） was detected by PCR

and the polymorphism was verified by sequencing. Results The frequency of distribution of II

ID and DD genotypes in the 95 patients was 44 cases（46. 32%）

38 cases（40%）

and 13 cases（13. 68%）

respectively. The control group was 33 cases（40. 24%）

40 cases（48. 78%）

9 cases（10. 98%）. The lung and kidney qi deficiency syndrome group was 16 cases（43. 24%）

17 cases（45. 95%）

and 4 cases（10. 81%）

respectively. The non-lung kidney qi deficiency group was 28 cases（48. 28%）

21 cases（36. 21%）

and 9 cases（15. 52%） respectively. There was no significant difference in the correlation analysis of the ACE gene SNP（I/D）（rs4646994） among the groups（P > 0. 05）. The results of the SNP sequence query showed that rs4646994 was an I/D mutation of a 289 bp repeat

but the known mutation frequency was less than 1%. Conclusion The ACE gene SNP（I/D）（rs4646994） is not enough evidence for the genetic markers of stable COPD of lung and kidney qi deficiency syndrome. Large samples are still needed for verification.

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