Objective To investigate the intervention effect and related mechanism of Shenfu Yixin Formula（ 参附益心方） on angiotensin Ⅱ（ AngⅡ）-induced H9c2 cardiomyocyte injury. Methods CCK-8 method was used to screen the concentration of Shenfu Yixin Formula

Coenzyme Q10 and Losartan potassium

and the concentration of AngⅡ was screened by MTT method. H9c2 cardiomyocytes were used as the research object

and the injury of H9c2 cardiomyocytes induced by AngⅡ was used as a model. The cells were divided into a normal control group

a model group

a coenzyme Q10 group

a losartan group and Shenfu Yixin Formula low and high dose groups. The normal control group was normally cultured for 24 hours. The model group was cultured for 24 hours with the culture medium containing the screening concentration of AngⅡ. The low-dose and high-dose groups of Shenfu Yixin Formula

the losartan group and the coenzyme Q10 group were cultured with the culture medium which were screened for the corresponding drug concentration and AngⅡ for 24 hours. The activity of H9c2 cardiomyocytes

mitochondrial content and membrane potential

reactive oxygen species（ ROS） content

apoptosis rate

expression of apoptosis-related factors Caspase-3

Caspase-8

Caspase-9

Bax

Bcl-2

Caspase-3

Caspase-8 and Caspase-9 protein activity were detected.Results Finally

the low-concentration and high-concentration of Shenfu Yixin Formula was 0. 25 and 0. 5 mg/ml respectively

the concentration of coenzyme Q10 was 1 × 10

-4

mol/L

and the concentration of losartan potassium was 1 × 10

-4

mol/L. The experiment of AngⅡ model was established with the concentration of 1 × 10

-5

mol/L. Compared with the normal control group

myocardial cell activity

mitochondrial number

membrane potential

Bcl-2 gene expression decreased in the model group. ROS content

apoptosis rate and expression of Caspase-3

Caspase-8

Caspase-9 and Bax genes increased. The activities of Caspase-3

Caspase-8 and Caspase-9 also increased（ P

<

0. 05）. Compared with the model group

the cell activity

mitochondrial number and membrane potential of Shenfu Yixin Formula high-dose group

losartan group and coenzyme Q10 group increased

ROS content

apoptosis rate

gene expression of Caspase-3

Caspase-9

Bax were decreased

and the activities of Caspase-3 and Caspase-9 also decreased（ P

<

0. 05）. Conclusion Shenfu Yixin Formula may reduce the mitochondrial function of cardiomyocytes and reduce the ROS content

thereby reducing the apoptosis of H9c2 cardiomyocytes induced by AngⅡ.