Objective To investigate the effects of Kangxian Yixin Formula（ 抗纤益心方） on norepinephrine（ NE）-induced apoptosis of H9c2 cardiomyocytes and its possible mechanism. Methods The optimal concentration of Kangxian Yixin Formula was screened by CCK-8 method. Rat H9c2 cardiomyocytes were cultured routinely

and cells were grouped after starvation for 8 h. The normal group was not treated. The model group was supplemented with 100 μmol/L NE 100 μl. The Kangxian Yixin Formula was added to the optimal concentration of Kangxian Yixin Formula solution 2 μl + 100 μmol/L NE 100 μl

captopril group 2 × 10

-5

mol/L captopril 5 μl + 100 μmol/L NE 100 μl

each group had 3 duplicate wells for 24 h. The apoptosis rate was detected by flow cytometry

and the expressions of apoptosis-related factors Bax

Bcl-2 and Caspase-3 mRNA and protein were detected. Results The final screening was performed at an intervention concentration of 0. 25 mg/ml Kangxian Yixin Formula. Compared with the normal group

the apoptosis rate of the model group increased

the expression of Bax and Caspase-3 mRNA and protein increased

and the expression of Bcl-2 mRNA and protein decreased（ P

<

0. 01）. Compared with the model group

the apoptosis rate of the Kangxian Yixin Formula group and the captopril group decreased

the expression of Bax and Caspase-3 mRNA and protein decreased

and the expression of Bcl-2 mRNA and protein increased（ P

<

0. 05 or P

<

0. 01）. Conclusion Kangxian Yixin Formula can inhibit NE-induced H9c2 cardiomyocyte apoptosis

and its mechanism may be related to the regulation of apoptosis-related factors Bax

Bcl-2 and Caspase-3.