Study on the Composition and Abundance of Intestinal Flora in Phlegm-Dampness Constitution Subjects Based on 16S rDNA Sequencing

JING Cai; XIAO Ning; ZHANG Xin; YU Huijuan; ZHANG Changyun; QU Yinuo; ZANG Xiaoming; MU Xiaoyi; TAN Qiwen

doi:10.13288/j.11-2166/r.2019.23.015

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Study on the Composition and Abundance of Intestinal Flora in Phlegm-Dampness Constitution Subjects Based on 16S rDNA Sequencing

更新时间：2021-11-10

- Study on the Composition and Abundance of Intestinal Flora in Phlegm-Dampness Constitution Subjects Based on 16S rDNA Sequencing
- Journal of Traditional Chinese Medicine Vol. 60, Issue 23, Pages: 2045-2049(2019)
- 作者机构：
  
  1. 山东中医药大学针灸推拿学院
  2. 山东中医药大学附属医院
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2019.23.015
  CLC： R229
- Published：2019
- 稿件说明：
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[1]景彩,肖宁,张昕,于慧娟,张昌云,曲一诺,臧晓明,母晓艺,谭奇纹.基于16S rDNA测序技术研究痰湿质受试者肠道菌群的组成及丰度[J].中医杂志,2019,60(23):2045-2049.

JING Cai, XIAO Ning, ZHANG Xin, et al. Study on the Composition and Abundance of Intestinal Flora in Phlegm-Dampness Constitution Subjects Based on 16S rDNA Sequencing[J]. Journal of Traditional Chinese Medicine2019, 60(23): 2045-2049.
[1]景彩,肖宁,张昕,于慧娟,张昌云,曲一诺,臧晓明,母晓艺,谭奇纹.基于16S rDNA测序技术研究痰湿质受试者肠道菌群的组成及丰度[J].中医杂志,2019,60(23):2045-2049. DOI： 10.13288/j.11-2166/r.2019.23.015.

JING Cai, XIAO Ning, ZHANG Xin, et al. Study on the Composition and Abundance of Intestinal Flora in Phlegm-Dampness Constitution Subjects Based on 16S rDNA Sequencing[J]. Journal of Traditional Chinese Medicine2019, 60(23): 2045-2049. DOI： 10.13288/j.11-2166/r.2019.23.015.

摘要

目的探讨痰湿质体质人群肠道菌群的丰度及物种组成差异。方法收集痰湿质及平和质受试者各30例粪便样本

提取两组样本中细菌总DNA进行检测定量

构建Miseq文库并测序

Miseq测序得到的PE读长根据重叠区关系进行拼接

再根据不同的相似度水平

对所有序列进行分类单元（OTU）聚类分析

并分别在各个分类水平上统计各样本的群落组成。利用16S rDNA测序技术分析痰湿质及平和质肠道菌群结构的组成及丰度变化。结果在分类单元（OTU）水平上

偏最小二乘法-判别分析（PLS-DA）显示痰湿质与平和质在肠道菌群组成上具有明显差异;在门水平上

痰湿质肠道菌群中拟杆菌门/厚壁菌门的比值低于平和质;在属水平上

痰湿质相对丰度下降的菌属主要为拟杆菌属、罗氏菌属（P <0. 01）、粪栖杆菌属、巨单胞菌

痰湿质相对丰度上升的菌属主要为戴阿利斯特菌属、Lachnoclostridium、直肠真杆菌属、Dorea。结论痰湿质与平和质体质受试者的肠道菌群在丰度及物种组成方面均有差异

其中拟杆菌门/厚壁菌门的比值降低以及罗氏菌属相对丰度的下降是痰湿质肠道菌群的特征。

Abstract

Objective To explore the abundance and species composition differences of intestinal flora in phlegm-dampness constitution people. Methods A total of 30 stool samples from phlegm-dampness constitution and 30 neutral constitution subjects were collected. The total bacterial DNA was extracted and quantified in the 2 groups. The Miseq library was constructed and the PE read length obtained by sequencing the Miseq sequencing was spliced according to the overlapping region relationship. According to different levels of similarity

operational taxonomic unit（ OTU） clustering analysis was performed on all sequences

and the community composition of each sample was counted at each classification level. 16 S rDNA sequencing technology was used to analyze the composition and abundance change of intestinal flora structure of the phlegm-dampness constitution and neutral constitution subjects.Results At the level of OTU

partial least squares discriminant analysis（ PLS-DA） showed that there was a significant difference in the composition of intestinal flora between the phlegm-dampness constitution and the neutral constitution; at the level of the phylum

the ratio of Bacteroides and Firmicutes in the phlegm-dampness constitution group was lower than that of the neutral constitution; at the genus level

the genus of the decreased relative abundance of phlegm-dampness constitution was mainly Bacteroides

Roche（ P < 0. 01）

Faecalibacterium and Megamonas

and the genus of increased relative abundance of phlegm-dampness constitution were mainly Dialister

Lachnoclostridium

Eubacterium rectale

and Dorea. Conclusion The intestinal flora of the subjects with phlegm-dampness constitution and neutral constitution is different in abundance and species composition

and the decreased ratio of Bacteroides and Firmicutes and the decreased relative abundance of Roche are the characteristics of the intestinal flora of phlegm-dampness constitution.

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