Objective To investigate the possible mechanism of type 2 diabetes treated by Banxia Xiexin Decoction（半夏泻心汤）. Methods MIN6 cells were divided into a blank group

a model group

a liraglutide group

and Banxia Xiexin Decoction low dose

medium dose and high dose group. Banxia Xiexin Decoction low dose

medium dose and high dose group were respectively added 10μl of Banxia Xiexin Decoction at concentration of 0.25

0.5

1.0 mg/ml pretreated for 12 h

and 0.18μg/ml Liraglutide Injection was added to the liraglutide group for 12 h. All groups of Banxia Xiexin Decoction

the liraglutide group and the model group used tert-butanol hydrogen peroxide（TBHP） to establish cell apoptosis model. MTT method was used to detect the cell apoptosis rate of each group

and Western blot method was used to detect the expression of cellular protein kinase B（AKT）

phosphorylated protein kinase B（p-AKT）

forkhead transcription factor 1（FOXO1）

phosphorylated forkhead transcription factor 1（p-FOXO1） and P27 protein. Results Compared with the blank group

the inhibition rate of MIN6 cells in the model group was significantly increased（P<0.05）. Compared with the model group

the inhibition rates of the liraglutide group and the Banxia Xiexin Decoction medium dose and high dose group were all reduced（P<0.05）. There was no significant difference between the intervention groups（P>0.05）). Compared with the blank group

the expression of p-AKT/AKT

p-FOXO1/FOXO1 in the model group was significantly down-regulated

and the expression of P27 was significantly up-regulated（P<0.05）. Compared with the model group

except p-FOXO1/FOXO1 in the Banxia Xiexin Decoction low dose group

the expression of p-AKT/AKT

p-FOX1/FOX1 in the liraglutide group and different dose of Banxia Xiexin Decoction groups were significantly up-regulated

and the expression of P27 was significantly down-regulated（P<0.05）. Compared with the Banxia Xiexin Decoction high-dose group

the expression of p-FOX1/FOX1 in the liraglutide group and the Banxia Xiexin Decoction medium-dose group was significantly up-regulated（P<0.05）. Compared with the Banxia Xiexin Decoction low-dose group

the expression of P27 in Banxia Xiexin Decoction medium-dose and high-dose group

and the liraglutide group was significantly down-regulated（P<0.05）. Conclusion The medium and high dose of Banxia Xiexin Decoction can significantly inhibit the apoptosis of MIN6 islet β cells to treat type 2 diabetes

and it is more effective at high doses. The mechanism may be activating the PI3 K/AKT/FOXO1 signaling pathway to regulate the expression of related proteins.